I-TAC is a “non-ELR” CXC chemokine that is regulated by interferon and signals through the CXCR3 receptor. I-TAC is chemoattractant for IL-2-activated T cells, but does not affect freshly isolated un-stimulated T cells, neutrophils, or monocytes. Recombinant Murine I-TAC is a 9.0 kDa protein containing 79 amino acid residues, including the four highly conserved cysteine residues present in CXC chemokines.
Amino Acid Sequence
Determined by its ability to chemoattract murine CXCR3-transfected HEK293 cells using a concentration range of 10.0–100.0 ng/ml.
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. This solution can be stored at 2–8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.