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Manufactured by BioVendor

ICAM-1 Human Chinese Hamster Ovary Cells (CHO)

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:Chinese Hamster Ovary Cells (CHO)
  • Other names:Intercellular adhesion molecule 1, Major group rhinovirus receptor, CD54 antigen, Surface antigen of activated B cells
  • Species:Human
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Cat. No. Size Price

RBG10180010 10 µg
RBG10180050 50 µg
RBG10180100 100 μg
PubMed Product Details
Technical Data


Recombinant protein


ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. ICAM-1 is a ligand for the lymphocyte function-associated antigen (LFA) and Mac-1 integrins, as well as the major human rhinovirus receptor. The primary function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human ICAM-1 gene codes for a 505 amino acid transmembrane glycoprotein containing a 29 amino acid cytoplasmic domain, a 23 amino acid transmembrane domain, and a 453 amino acid extracellular domain. Recombinant Human ICAM-1 is a 49.5 kDa glycoprotein comprising the extracellular domain (453 amino acid residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of approximately 72.0–80.0 kDa by SDS-PAGE analysis under reducing conditions.

Amino Acid Sequence



Chinese Hamster Ovary Cells (CHO)



Biological Activity

ICAM-1 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at 25 µg/ml ICAM-1 concentration.


Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).


Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at-20°C to –80°C.




Research topic

Cell adhesion proteins


ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFNγ within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR. The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations. ICAM-1 upregulation in allergic airway inflammation is responsible for the recruitment of activated leukocytes and the pathogenesis of allergic rhinitis.

Summary References (19)

References to ICAM-1

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  • Adams DH, Mainolfi E, Elias E, Neuberger JM, Rothlein R. Detection of circulating intercellular adhesion molecule-1 after liver transplantation--evidence of local release within the liver during graft rejection. Transplantation. 1993 Jan;55 (1):83-7
  • Altomonte M, Colizzi F, Esposito G, Maio M. Circulating intercellular adhesion molecule 1 as a marker of disease progression in cutaneous melanoma. N Engl J Med. 1992 Sep 24;327 (13):959
  • Arocker-Mettinger E, Steurer-Georgiew L, Steurer M, Huber-Spitzy V, Hoelzl E, Grabner G, Kuchar A. Circulating ICAM-1 levels in serum of uveitis patients. Curr Eye Res. 1992;11 Suppl:161-6
  • Becker JC, Dummer R, Schwinn A, Hartmann AA, Burg G. Circulating intercellular adhesion molecule-1 in melanoma patients: induction by interleukin-2 therapy. J Immunother (1991). 1992 Aug;12 (2):147-50
  • Dustin ML, Rothlein R, Bhan AK, Dinarello CA, Springer TA. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J. Immunol. 1986. 137: 245-254. J Immunol. 2011 May 1;186 (9):5024-33
  • Harning R, Mainolfi E, Bystryn JC, Henn M, Merluzzi VJ, Rothlein R. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res. 1991 Sep 15;51 (18):5003-5
  • Lal RB, Mainolfi E, Rothlein R. Elevated levels of cICAM-1 in patients with human T-cell leukemia virus type I associated myelopathy and adult T-cell leukemia. Blood. 1992 Nov 1;80 (9):2434-5
  • Lampeter ER, Kishimoto TK, Rothlein R, Mainolfi EA, Bertrams J, Kolb H, Martin S. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes. 1992 Dec;41 (12):1668-71
  • Lisby S, Ralfkiaer E, Rothlein R, Vejlsgaard GL. Intercellular adhesion molecule-I (ICAM-I) expression correlated to inflammation. Br J Dermatol. 1989 Apr;120 (4):479-84
  • Marlin SD, Springer TA. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell. 1987 Dec 4;51 (5):813-9
  • Most J, Zangerle R, Herold M, Fuchs D, Wachter H, Fritsch P, Dierich MP. Elevated concentrations of circulating intercellular adhesion molecule 1 (ICAM-1) in HIV-1 infection. J Acquir Immune Defic Syndr. 1993 Mar;6 (3):221-6
  • Pober JS, Gimbrone MA Jr, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. J Immunol. 1986 Sep 15;137 (6):1893-6
  • Potocnik AJ, Kinne R, Menninger H, Zacher J, Emmrich F, Kroczek RA. Expression of activation antigens on T cells in rheumatoid arthritis patients. Scand J Immunol. 1990 Feb;31 (2):213-24
  • Rothlein R, Czajkowski M, O'Neill MM, Marlin SD, Mainolfi E, Merluzzi VJ. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies. J Immunol. 1988 Sep 1;141 (5):1665-9
  • Rothlein R, Dustin ML, Marlin SD, Springer TA. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J. Immunol. 1986. 137: 1270-1274. J Immunol. 2011 May 1;186 (9):5034-8
  • Rothlein R, Mainolfi EA, Czajkowski M, Marlin SD. A form of circulating ICAM-1 in human serum. J Immunol. 1991 Dec 1;147 (11):3788-93
  • Salafia CM, DeVore GR, Mainolfi E, Kelly J, Pezzullo JC, Rothlein R. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am J Obstet Gynecol. 1993 Oct;169 (4):830-4
  • Vejlsgaard GL, Ralfkiaer E, Avnstorp C, Czajkowski M, Marlin SD, Rothlein R. Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin les
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