ID3EAL Cancer miRNA Panel

Four distinct cell types (HDFa, BE2C, HCT116 and U251) were profiled with the ID3EAL Cancer miRNA Panel. One hundred nanograms of RNA from each sample was used for reverse transcription. About 300 miRNAs were detected for each cell line, with 244 common miRNAs detected with CT ≤ 33 across all 4 cell lines.

11056

Number of miRNAs Detected CT number of miRNAs Detected

Inter-plate calibrator normalization and biological normalization by global mean or reference miRNAs were performed prior to inter-sample miRNA ΔCT calculation. MiRNA expression comparison in HFDa and U251 cells are visualized through a scatter plot. Data points that fall outside of ±1 cycles regression lines indicate miRNAs differentially expressed in the both cell lines.

11049

Format of Panel

11051

Cancer miRNA knowledge panel

CAT. NO.	Name	DESCRIPTION	SAMPLES
1105152	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 6 x 96-well PCR plates	6
1105153	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 12 x 96-well PCR plates	12
1105154	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates	24
1105262	ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (6)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates	6
1105263	ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (12)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 48 x 96-well PCR plates	12
1105264	ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (24)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 96 x 96-well PCR plates	24

Choose your thermal cycler

Roche
Abi Fast
Abi Standard
Bio-Rad

Cancer miRNA Panel Analysis Template