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ID3EAL Cancer miRNA Panel

Four distinct cell types (HDFa, BE2C, HCT116 and U251) were profiled with the ID3EAL Cancer miRNA Panel. One hundred nanograms of RNA from each sample was used for reverse transcription. About 300 miRNAs were detected for each cell line, with 244 common miRNAs detected with CT ≤ 33 across all 4 cell lines.

11056
Number of miRNAs Detected                                                  CT number of miRNAs Detected

Inter-plate calibrator normalization and biological normalization by global mean or reference miRNAs were performed prior to inter-sample miRNA ΔCT calculation. MiRNA expression comparison in HFDa and U251 cells are visualized through a scatter plot. Data points that fall outside of ±1 cycles regression lines indicate miRNAs differentially expressed in the both cell lines.


11049

Format of Panel

11051

 Cancer miRNA knowledge panel 

CAT. NO. Name DESCRIPTION SAMPLES
1105151 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
2 x 96-well PCR plates
2
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105261 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (2) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
8 x 96-well PCR plates
2
1105262 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
6
1105263 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
12
1105264 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
96 x 96-well PCR plates
24

Choose ID3EAL Cancer Knowledge Panel

  • Cancer Panel A (HSA01111A)
  • Cancer Panel B (HSA01112A)
  • Cancer Panel C (HSA01113A)
  • Cancer Panel D (HSA01114A)
List of targets you can download here: Plate layout

 

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Cancer miRNA Panel Analysis Template