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ID3EAL Stem Cell miRNA Panel

In this study, the research group aims to profile and identify key microRNA targets driving the morphological changes between bone marrow and adipose lineages from Human Fetal MSCs. Human fetal MSCs were cultured (no more than 7th passage). Cell line specific factors were introduced to differentiate them into bone marrow and adipose lineages for 6 days. Five biological replicates from each group were collected and profiled with ID3EAL Stem Cell miRNA knowledge panel. 


10993

 

One hundred nanograms of total RNA from each sample was used for reverse transcription and qPCR profiling. Of the 176 miRNAs, 165 miRNAs were detected in adipose lineage differentiation and 164 miRNAs were detected in cells from bone marrow lineages.

Most significantly regulated miRNAs can be identified through the examination of p-value and 2-ΔΔCT fold change. Candidate miRNAs with most significant p-values and fold changes can be visualized through a volcano plot (Figure 5). MiRXES offers wet lab validated individual miRNA qPCR assays for further validation of identified miRNA candidate.

10994
Single out potential key microRNA regulators

Format of Panel

 

11051

Stem Cell

CAT. NO. Name DESCRIPTION SAMPLES
1105151 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
2 x 96-well PCR plates
2
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105251 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (2) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
4 x 96-well PCR plates
2
1105252 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
6
1105253 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
12
1105254 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
24

Choose ID3EAL Stem Cell Knowledge Panel

  • Stem Cell Panel A (HSA01106A)
  • Stem Cell Panel B (HSA01107A)
List of targets you can download here: Plate layout

 

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad
Stem Cell miRNA Panel Analysis Template