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Summary

IHS Buffer

6705Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from:

  • non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often “affinity purified” means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions)
  • low specificity antibodies among specific ones in polyclonal
  • fragments of fallen apart IgGs in stored preparations, including monoclonal
  • separate heavy and light chains of specific antibodies, produced by most hybridomas

All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In case of retrieved formalin sections the risk of non-specific reaction is even increased, since to active the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making accessible many domains that are charged and are capable of binding the test immunoglobulins on non-specific manner.

The standard means to block non-specific binding of specific antibody preparation is to add some irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who has attempted to do this knows that increasing (for effective blocking) concentration of such blocking agents leads to a great reduction of specific reaction as well. This is due to large blocking molecules binding to accessible sites on sections and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). Instead of these proteins, all of our buffers developed for immune assays contain short (0.6–2 kD) peptides that are capable of effectively blocking non-specific reactions while not affecting the specific binding of antibody.

Immunohistology buffers

Low background and strong specific reaction. Standardized staining.

Aptum's collection of Immunohistology buffers also has some other benefits (see below) and allows you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, hybridization of sections with antibody detection. The Retriever IHC buffers empower you to control non-specific staining on every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.

All buffers available in 50 ml, 125 ml and 500 ml package. Ready to use. Certified for use in diagnostic applications.

Section Block

A new class of blocking solutions are based on chemically modified and fragmented ultra-pure casein. This effectively reduces unwanted binding of primary antibody and conjugates you use to charged surfaces of the slide and tissue section, and it greatly reduces non-specific binding while preserving the specific reaction, by saturating potential non-specific protein-protein interactions. Moreover, in contrast to BSA-based, IgGm casein or serum -based blocking solutions there is no interaction of specific antibody and blocking protein itself. It is not comparable to other commercilally available or home-made blocking solutions. Recommended for research and diagnostic pathology, especially for retrieved sections and polyclonal antibodies.

HRP-Conjugate Diluent

This diluent is specifically designed for preparing solutions of your HRP-conjugate used as the detection reagent. It is the Antibody-diluent buffer with additional components for stabilising your HRP-conjugate. It allows you to further standardise the assay by preparing ready-to-use conjugate solutions in advance ans store them in a refrigerator without loss of activity.

Antibody Diluent

Buffer for diluting your primary and secondary antibodies, especially if they were stored for longer periods of time, even at –20°C in glycerol, or in refrigerator. Nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies will be minimised, making your result more reliable. Excellent for IHC (frozen and formalin sections), flow cytometry on fixed cells, Western Blot and other immune assays.

When used in pathology, in also greatly reduces non-specific reactivity of human serum components and immunoglobilins in tissue, vessels and cells with mouse antibodies used on section.

For especially “troublesome“ antibodies, as well as for in situ PCR applications, this diluent may also be used as a washing buffer, preventing secondary binding of your analystes during washing.

 

Product Size Price
1.5mm

Type: Puncher Tip

TAT06-1.5 1 ea
 
1.5mm (10 x 15 ; 150 wells)

Type: Mold Kit

TAM01-1.5 1 ea
 
1.5mm (9 x 10 ; 90 wells)

Type: Premade Recipient Block

TAB06-1.5 1 ea
 
1mm

Type: Puncher Tip

TAT06-1 1 ea
 
1mm (10 x 12 ; 120 wells)

Type: Premade Recipient Block

TAB06-1 1 ea
 
1mm (10 x 17 ; 170 wells)

Type: Mold Kit

TAM01-1 1 ea
 
2mm

Type: Puncher Tip

TAT06-2 1 ea
 
2mm (6 x 10 ; 60 wells)

Type: Premade Recipient Block

TAB06-2 1 ea
 
2mm (7x 10 ; 70 wells)

Type: Mold Kit

TAM01-2 1 ea
 
3mm

Type: Puncher Tip

TAT06-3 1 ea
 
3mm (5 x 6 ; 30 wells)

Type: Premade Recipient Block

TAB06-3 1 ea
 
3mm (5 x 8 ; 40 wells)

Type: Mold Kit

TAB01-3 1 ea
 
5mm

Type: Puncher Tip

TAT06-5 1 ea
 
5mm (3 x 5 ; 15 wells)

Type: Mold Kit

TAB-01-5 1 ea
 
5mm (4 x 5 ; 20 wells)

Type: Premade Recipient Block

TAB06-5 1 ea
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-050 50 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-125 125 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-500 500 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0742-050 50 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-125 125 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-500 500 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-050 50 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-125 125 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-500 500 ml
 
Base mold

Type: Mold Kit

TABM06 1 ea
 
Basket

Type: Accessories

R21008 1 each
 
Basket Lid for Chambers

Type: Accessories

R21009 1 each
 
Coating Buffer 7.4

Type: ELISA

AP0511-500 500 ml
 
Coating Buffer 9.6

Type: ELISA

AP0510-500 500 ml
 
Cord Set EU

Type: Accessories

R21005 1 each
 
Embedding cassette

Type: Mold Kit

TAC06 500/PK
 
Full set with 5 Tips and 5 sized blocks

Type: Tissue Microarrayer Set

TAT06 1 set
 
Green Silicone Sealing Gasket

Type: Accessories

R21007 1 each
 
Guide for 1mm recipient block

Type: Puncher Tip

TAG06 1 set
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-050W 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-125W 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-500W 500 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-050E 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-125E 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-500E 500 m
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-050 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-125 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-500 500 ml
 
Plate Block E

Type: ELISA

AP0513-500 500 ml
 
Plate Block, No Components of Animal Origin

Type: ELISA

AP0512-125 125 ml
 
Retriever Unit

Type: Retriever Unit

R2100-EU 1 each
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-050 50 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-125 125 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-500 500 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-050 50 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-125 125 ml
 
Single set for 1 mm puncher with a guide

Type: Tissue Microarrayer Set

TAT06T1 1 set
 
Single set for 1.5mm puncher

Type: Tissue Microarrayer Set

TAT06T1.5 1 set
 
Single set for 2mm puncher

Type: Tissue Microarrayer Set

TAT06T2 1 set
 
Single set for 3mm puncher

Type: Tissue Microarrayer Set

TAT06T3 1 set
 
Single set for 5mm puncher

Type: Tissue Microarrayer Set

TAT06T5 1 set
 
Slide Chamber

Type: Accessories

R21001 Set of 3
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-050 50 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-125 125 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-500 500 ml
 
Universal Buffer

Type: Universal Buffers

AP0530-125 125 ml, 10x
 
Universal Buffer

Type: Universal Buffers

AP0530-500 500 ml, 10x
 
Washing Buffer E PBS-Based 10x

Type: ELISA

AP0515-500 500 ml
 
Washing Buffer W Tris-Based 10x

Type: Western Blot Buffers

AP0502-500 500 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-125 125 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-500 500 ml