Notice
For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml).
Please find the protocol for preparation and analysis of stool extracts in Docs.
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Stool, Serum, Plasma
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
31.25 – 2000 pg/ml
Limit of Detection
13 pg/ml
Intra-assay (Within-Run)
n = 8, CV = 3%
Inter-assay (Run-to-Run)
n = 6, CV = 4.6%
Spiking Recovery
Serum: 96.6%
Stool: 101.93%
Dilution Linearity
Serum: 102.5.6%
Stool: 110%
Features
- The total assay time is less than 3.5 hours
- The kit measures FABP6 protein in human serum, plasma (EDTA, citrate, heparin) and stool samples
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Oncology, Others, Reproduction
Summary
FABP6 is protein from family fatty acid-binding proteins (FABPs). FABP6 is highly expressed in the enterocytes of ileum and to a lesser extent in the luteal cells of ovaries, placenta and adrenal glands. FABP6 acts as an intracellular transporter of bile acids in ileal epithelial cells, helping to metabolize cholesterol. FABP6 plays a significant role in granulosa cells differentiation required for follicular rupture.
FABP6 is potential biomarker for various cancers and it may be useful clinically in cancer diagnosis and for the assessment of cancer therapy. The expression of FABP6 was higher in primary colorectal cancers and adenomas than in normal epithelium, but was dramatically decreased in lymph node metastases. Tumors with high FABP6 expression were more frequently located in left colon than the right colon and had shallower invasion into the bowel wall. Tumors with high expression FABP6 were smaller in size. Increase FABP6 expression were described for prostate, bladder and renal cell carcinoma.
Instructions for Use (RUO)
Application protocol
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
Find documents for the lot