Ileal FABP (FABP6) Human ELISA

Cat. No.	Size	Price
Availability on Request RD191248200R	96 wells (1 kit)

Technical Data

Notice

For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml).

Please find the protocol for preparation and analysis of stool extracts in Docs.

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Stool, Serum, Plasma

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

31.25 – 2000 pg/ml

Limit of Detection

13 pg/ml

Intra-assay (Within-Run)

n = 8, CV = 3%

Inter-assay (Run-to-Run)

n = 6, CV = 4.6%

Spiking Recovery

Serum: 96.6%
Stool: 101.93%

Dilution Linearity

Serum: 102.5.6%
Stool: 110%

Summary

Features

The total assay time is less than 3.5 hours
The kit measures FABP6 protein in human serum, plasma (EDTA, citrate, heparin) and stool samples
Assay format is 96 wells
Standard is recombinant protein based
Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Oncology, Others, Reproduction

Summary

FABP6 is protein from family fatty acid-binding proteins (FABPs). FABP6 is highly expressed in the enterocytes of ileum and to a lesser extent in the luteal cells of ovaries, placenta and adrenal glands. FABP6 acts as an intracellular transporter of bile acids in ileal epithelial cells, helping to metabolize cholesterol. FABP6 plays a significant role in granulosa cells differentiation required for follicular rupture.

FABP6 is potential biomarker for various cancers and it may be useful clinically in cancer diagnosis and for the assessment of cancer therapy. The expression of FABP6 was higher in primary colorectal cancers and adenomas than in normal epithelium, but was dramatically decreased in lymph node metastases. Tumors with high FABP6 expression were more frequently located in left colon than the right colon and had shallower invasion into the bowel wall. Tumors with high expression FABP6 were smaller in size. Increase FABP6 expression were described for prostate, bladder and renal cell carcinoma.

Product References (1)

References

Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K. The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males. Antioxidants (Basel). 2021 May 28;10(6):866. doi: 10.3390/antiox10060866. PubMed PMID: 34071378. PubMed CentralPMCID: PMC8229363. See more on PubMed

Summary References (8)

References to Gastrotropin (FABP6)

Collado-Romero M, Aguilar C, Arce C, Lucena C, Codrea MC, Morera L, Bendixen E, Moreno A, Garrido JJ: Quantitative proteomics and bioinformatics analysis provide new insight into dynamic response of porcine intestine to Salmonella Typhimurium. J of Frontiers in Cellular and Infection Microbiology. September; 5 (64): (2015)
Duggavathi R, Siddappa D, Schuermann Y, Pansera M, Menard IJ, Praslickova D, Agellon LB: The fatty acid binding protein 6 gene (Fabp6) is expressed in murine granulosa cells and is involved in ovulatory response to superstimulation. J of Reproduction and Development. March; 61 (3): 237 – 240 (2015)
Ohmachi T, Inoue H, Mimori K, Tanaka F, Sasaki A, Kanda T, Fujii H, Yanaga K, Mori M: Fatty Acid Binding Protein 6 Is Overexpressed in Colorectal Cancer. J of Clinical Cancer Research. September; 12 (17): 5090 – 5095 (2006)
Ono T, Odani S: Initial studies of the cytoplasmic FABP superfamily. J of Proceedings of the Japan Academy Series B. 86 (3): 220 – 228 (2010)
Praslickova D, Torchia EC, Sugiyama MG, Magrane EJ, Zwicker BL, Kolodzieyski L, Agellon LB: The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine. J of PLOS ONE. December; 7 (22): e50810 (2012)
Smathers RL, Petersen DR: The human fatty acid-binding protein family: Evolutionary divergenes and functions. J of Human Genomics. March; 5 (3): 170 – 191 (2011)
Tölle A, Suhail S, Jung M, Jung K, Stephan C: Fatty acid binding proteins (FABPs) in prostate, bladder and kidney cancer cell lines and the use of IL-FABP as survival predictor in patients with renal cell carcinoma. J of BMC Cancer. July; 11 (302): (2011)
Uribe JH, Collado-Romero M, Zaldívar-López S, Arce C, Bautista R, Carvajal A, Cirera S, Claros MG, Garrido: Transcriptional analysis of porcine intestinal mucosa infected with Salmonella Typhimurium revealed a massive inflammatory response and disruption of bile acid absorption in ileum. J of Veterinary Research. January; 47 (11): (2016)

Docs

Instructions for Use (RUO)

Application protocol
Instructions for Use (RUO)

Safety Information (RUO)

MSDS (RUO)