Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Insulin Receptor Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:INSR Protein, IR, short isoform (HIR-A, IR-A), CD220
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RD191041200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma (EDTA, citrate, heparin)

Sample Requirements

30 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.47–30 ng/ml

Limit of Detection

0.173 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 5.2 %

Inter-assay (Run-to-Run)

n = 5; CV = 4.0 %

Spiking Recovery

99.3 %

Dilutation Linearity

101.2 %

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 4.5 hours
  • The kit measures total insulin receptor in human serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Diabetology - Other Relevant Products, Diabetology - Insulin, C-Peptide, Proinsulin, Energy metabolism and body weight regulation, Oncology

Summary

Insulin receptor (IR) is an α2β2-disulfide linked tetrameric tyrosin kinase receptor located in the plasma membrane of target cells. This glycoprotein is composed of two extracellular α-subunits (731 amino acids; 135 kDa) containing the insulin binding site and two transmembrane β-subunits (620 amino acids; 95kDa) that possess intrinsic tyrosine kinase activity in their intracellular domains and transduce the insulin signal into the cell interior. The human insulin receptor is involved in glucose homeostasis, cell growth and differentiation. Binding of insulin leads to a conformational change of the receptor, resulting in ATP binding, autophosphory­lation, and subsequent phosphorylation of insulin receptor substrate proteins that are linked to the action of two main signalling pathways. The PI3-K/Akt pathway is involved in the glucose transport to the cell, induction of proliferation or inhibition of apoptosis, while the Ras/MAPK pathway is involved mainly in the control of cell growth and differentiation. Two insulin receptor variants are produced in mammals by alternative splicing: IR-A lacking exon 11 and the full length IR-B. The IR-A and IR-B isoforms show different ligand binding affinity. IR-A is a high-affinity receptor not only for insulin but also for IGF-II, while IR-B may be considered a specific receptor for insulin. Both insulin receptor isoforms are coexpressed in cells, and the relative abundance of IR-A and IR-B is regulated by development stage- and tissue-specific factors. IR-A is predominantly expressed in fetal and cancer cells, whereas IR-B is predominantly expressed in well-differentiated tissues including liver, adipose tissue and skeletal muscle. Dysregulation of insulin receptor splicing, i.e., increased IR-A expression in adult life, may play an underestimated role in cancer progression. Insulin receptor is overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas. Moreover, human lymphocyte-derived malignant cells, such as the IM-9 cells, are abundantly endowed with high-affinity insulin receptors. Circulating forms of several classes of receptor molecules and their fragments have been identified in human plasma. The human insulin receptor was found to be secreted into the incubation medium by various cultured cell lines and Schaefer et al. reported that transgenic mice expressing and secreting the soluble ectodomain of human insulin receptor into the plasma showed chronic hyperglycemia. Another study has shown that injection of the purified His-tagged human insulin receptor α-subunit into veins of mice increased in the concentration of blood glucose. The soluble human insulin receptor ectodomain, which contains α-subunit and a extracellular part of β-subunit, has been observed in human plasma of healthy individuals and observed at significantly elevated levels in plasma of patients with elevated blood glucose. Furthermore, the urinary soluble insulin receptor levels in patients with diabetes were also significantly higher than those in healthy volunteers and were significantly correlated with both urinary resistin and insulin levels.

References to Summary

References to Insulin Receptor

  • Belfiore A, Frasca F, Pandini G, Sciacca L, Vigneri R. Insulin receptor isoforms and insulin receptor/insulin-like growth factor receptor hybrids in physiology and disease. Endocr Rev. 2009 Oct;30 (6):586-623
  • Giudice J, Jares-Erijman EA, Leskow FC. Insulin receptor membrane retention by a traceable chimeric mutant. Cell Commun Signal. 2013;11 (1):45
  • Kanezaki Y, Matsushima R, Obata T, Nakaya Y, Matsumoto T, Ebina Y. Injection of the insulin receptor alpha subunit increases blood glucose levels in mice. Biochem Biophys Res Commun. 2003 Sep 26;309 (3):572-7
  • Malaguarnera R, Belfiore A. The insulin receptor: a new target for cancer therapy. Front Endocrinol (Lausanne). 2011;2:93
  • Papa V, Russo P, Gliozzo B, Goldfine ID, Vigneri R, Pezzino V. An intact and functional soluble form of the insulin receptor is secreted by cultured cells. Endocrinology. 1993 Sep;133 (3):1369-76
  • Sacco A, Morcavallo A, Pandini G, Vigneri R, Belfiore A. Differential signaling activation by insulin and insulin-like growth factors I and II upon binding to insulin receptor isoform A. Endocrinology. 2009 Aug;150 (8):3594-602
  • Schaefer EM, Viard V, Morin J, Ferre P, Penicaud L, Ramos P, Maika SD, Ellis L, Hammer RE. A new transgenic mouse model of chronic hyperglycemia. Diabetes. 1994 Jan;43 (1):143-53
  • Taniguchi CM, Emanuelli B, Kahn CR. Critical nodes in signalling pathways: insights into insulin action. Nat Rev Mol Cell Biol. 2006 Feb;7 (2):85-96
  • The Soluble Insulin Receptor Study Group. Soluble insulin receptor ectodomain is elevated in the plasma of patients with diabetes. Diabetes. 2007 Aug;56 (8):2028-35
  • Umehara A, Nishioka M, Obata T, Ebina Y, Shiota H, Hashida S. A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus. Clin Biochem. 2009 Sep;42 (13-14):1468-75
Related Products Documents