Total 927 AA, UniProt P06213–2 (His28-Lys944 of HIR-A, whole subunit α and extracellular domain of subunit β). MW: 105.9 kDa (calculated), migrates at ~ 160 kDa on SDS PAGE. N-terminal linker (2 extra AA), C-terminal linker (2 extra AA) and C-terminal His-tag (6 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: >95%
8% SDS-PAGE separation of Human INSR Protein: 1. M.W. marker – 45, 66, 97 kDa 2. reduced and heated sample, 2.5μg/lane 3. non-reduced and non-heated sample, 2.5μg/lane
Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4
Add 100ul of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Western blotting, ELISA, Cell culture and/or animal studies
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.