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Interleukin-10 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-10, Cytokine synthesis inhibitory factor, B cell derived T cell growth factor, Mast cell growth factor III), Insulin receptor substrate (IRS)
  • Species:Human
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Cat. No. Size Price


RAF026R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Summary

Research topic

Cytokines and chemokines and related molecules

Summary

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells
and thymocytes. The primary structure of Human IL-10 has been determined by cloning the cDNA encoding
the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution Human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The Human IL-10 gene is located on chromosome 1 and is present as a single copy
in the genome. The Human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.
IL-10 in disease: IL-10 expression was shown to be elevated in parasite infections like in Schistosoma mansoni, Leishmania, Toxoplasma gondii and Trypanosoma infection. Furthermore, high IL-10 expression was detected in mycobacterial infections as shown for Mycobacterium leprae, Mycobacterium tuberculosis and Mycobacterium avium infections. High expression levels of IL-10 are also found in retroviral infections inducing
immunodeficiency.

References to Product

References

  • Tsigou E, Stavros A, Pavlos M, Stavros G, Athanassios T, George B. The immune response after stimulation with wall components of gram-positive bacteria and fungi. Immunol Lett. 2014 May-Jun;159 (1-2):23-9
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