Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Interleukin-2 Murine E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:IL-2, Aldesleukin, T cell growth factor, lymphokine
  • Species:Murine
Please select your region to see available products and prices.
Cat. No. Size Price


RBG20002005 5 µg
RBG20002020 20 µg
RBG20002100 100 μg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant Murine IL-2 is a 17.2 kDa protein containing 148 amino acid residues.

Amino Acid Sequence

PTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

Source

E. coli

Purity

98%

Biological Activity

The ED50 as determined by the dose dependent stimulation of murine CTLL-2 cells is 0.2 ng/ml, corresponding to a specific activity of 5×106 units/mg.

Endotoxin

Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. This solution can be stored at 2–8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.

Storage/Expiration

 –20°C

Summary

Research topic

Cytokines and chemokines and related molecules, Animal studies

Summary

Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. IL-2 gene expression is regulated at the transcriptional level by several activation pathways. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors for IL-2 induced in an autocrine fashion on the surface of T-cells. Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon- and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. Alterations in the ability of T-cells to synthesize IL-2 have been observed in physiologic and pathologic states.

Related Products Docs