Total 169 AA. MW: 19,2 kDa (calculated). UniProtKB acc.no. O95760. N-Terminal His-tag, 10 extra AA (highlighted).
Amino Acid Sequence
14% SDS-PAGE separation of Human Interleukin-33
1. reduced and heated sample, 5μg / lane
2. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
3. non-reduced and non-heated sample, 5μg / lane
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.