Sandwich ELISA, Biotin-labelled antibody
Stool, Serum, Plasma-EDTA, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Milk, Saliva
Serum, Plasma (EDTA), BALF, Breast milk, Saliva: 5 µl/well
CSF, Urine, Stool extract: 20 µl/well
- For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml)
- Special Dilution Buffer is needed for measurement of human lactoferrin in stool extract and is not included.
For protocol for preparation of stool extracts and other details, please contact us at firstname.lastname@example.org
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box)
Limit of Detection
n = 8; CV = 3.3 %
n = 6; CV = 5.4 %
Serum: 96.6 %
Plasma (EDTA): 103.2 %
BALF: 104.7 %
CSF: 102.3 %
Urine: 104.4 %
Stool extract: 89.7 %
Serum: 94.6 %
Plasma (EDTA): 97.5 %
BALF: 88.3 %
CSF: 99.5 %
Urine: 98.4 %
Breast milk: 97.1 %
Saliva: 97.1 %
Stool extract: 94.9 %
- It is intended for research use only
- The total assay time is less than 3.5 hours
- The kit measures lactoferrin in serum, plasma (EDTA), bronchoalveolar lavage fluid (BALF), cerebrospinal fluid (CSF), urine, breast milk, saliva and stool extract
- Special Dilution Buffer is needed for measurement of human lactoferrin in stool extract and is not included. For protocol for preparation of stool extracts and other details, please contact us at email@example.com
- Assay format is 96 wells
- Standard is native protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Bone and cartilage metabolism, Immune Response, Infection and Inflammation, Inflammatory bowel disease, Iron metabolism
Lactoferrin is 703-amino acid iron-binding glycoprotein that belongs to the transferrin family. Lactoferrin capability of binding iron is two times higher than that of transferrin. Two ferric ions can be bound by one lactoferrin molecule. There are three forms of lactoferrin according to its iron saturation: apolactoferrin (iron free), monomeric lactoferrin (one ferric ion), and hololactoferrin (binds two Fe3+ ions). The tertiary structure of hololactoferrin and apolactoferrin is different.
Lactoferrin was originally isolated from breast milk and subsequently was identified in secretions from exocrine glands and in specific granules of neutrophils. Neutrophils after degranulation are main source of lactoferrin in blood plasma. Lactoferrin has been found in most mucosal secretions such as uterine fluid, saliva, bile, pancreatic juice, small intestine secretions, nasal secretion, and tears. Lactoferrin is also present in urine and fecal samples, though levels in these samples are relatively low. The kidney produces lactoferrin in a highly ordered manner but only a minor fraction of the protein is secreted into urine. The biological properties of lactoferrin are mediated by specific receptors on the surface of target cells and can be found, for example, on mucosal epithelial cells, hepatocytes, monocytes, macrophages, polymorphonuclear leukocytes, lymphocytes, thrombocytes, fibroblasts, and on some bacteria.
Lactoferrin possesses various biological functions including its roles in iron metabolism, cell proliferation and differentiation, and antibacterial, antiviral, and antiparasitic activity. Many of these functions do not appear to be connected with its iron binding ability. During most inflammatory reactions lactoferrin concentration increases in all biological fluids, and several authors classify lactoferrin as an acute-phase protein. However, the relationship between its concentration and physiological or pathological effects on body functions is not yet well characterized. Lactoferrin has even been reported to inhibit the development of experimental metastases in mice. Lactoferrin has also been identified as a potent anabolic factor affecting osteocytes, where it induces osteoblast proliferation, survival, differentiation, reduces apoptosis of osteoblasts by 50-70% and inhibits osteoclast formation. The influence of lactoferrin on lipid metabolism was discovered through animal studies. Oral administration of bovine lactoferrin reduced plasma cholesterol levels and retarded hepatic lipid accumulation in mice and decreased serum TAG to 72% of the control level in rats. Another study of abdominally obese men and women showed that ingestion of lactoferrin reduced visceral fat. Fecal lactoferrin level has investigated for its use as a non-invasive marker in the distinction of inflammatory bowel disease (IBD) and non-inflammatory condition.