UniProtKB acc.no. P41160
Amino Acid Sequence
>98% by SDS PAGE gel analysis
14% SDS-PAGE separation of Mouse Leptin
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane
Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
< 1.0 EU/ug
Filtered (0.4 μm) and lyophilized with 0.1% TFA.
Add injection water to prepare a working stock solution of 1.0 mg/mL and let the lyophilized pellet dissolve completely. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store at –80°C. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.