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M371-test for Testicular Cancer

  • Regulatory status:RUO
  • Type:RT-qPCR diagnostic kit
  • Species:Human
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Cat. No. Size Price


New MCS0105 5 samples + 5NC
New MCS0115HT 15 samples + 1 NC
PubMed Product Details
Technical Data

Type

RT-qPCR diagnostic kit

Description

The laboratory diagostic M371-Test is an in vitro diagsnotic (IVD) method based on the measurement of the relative abundance (RQ) of the tumor marker miR-371a-3p. For this purpose, miR-371a-3p and an endogenous control are quantified in 200ml of serum from the cobital vein by qPCR.

Applications

Serum

Sample Requirements

200 µl of serum

Shipping

Shipment at -25 to -15°C.

Storage/Expiration

Store the complete kit at -23 to -15 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Summary

Features

  • Diagnostic kit is certified for in vitro diagnostic.
  • The assay specificity is 94% ans sensitivity is 98,4%.
  • Includes the internal control.
  • Kit does not include RNA extraction kit.

Research topic

Oncology, Reproduction, Serology

Summary

The M371-Test is a diagnostic kit with a qualitative sample interpretation that detects the presence of testicular germ cell tumors (TGCT) and can be used for diagnosis and follow-up monitoring of this tumor by expert operators.

Product References (1)

References

  • Dieckmann KP, Radtke A, Geczi L, Matthies C, Anheuser P, Eckardt U, Sommer J, Zengerling F, Trenti E, Pichler R, Belz H, Zastrow S, Winter A, Melchior S, Hammel J, Kranz J, Bolten M, Krege S, Haben B, Loidl W, Ruf CG, Heinzelbecker J, Heidenreich A, Cremers JF, Oing C, Hermanns T, Fankhauser CD, Gillessen S, Reichegger H, Cathomas R, Pichler M, Hentrich M, Eredics K, Lorch A, Wülfing C, Peine S, Wosniok W, Bokemeyer C, Belge G. Serum Levels of MicroRNA-371a-3p (M371 Test) as a New Biomarker of Testicular Germ Cell Tumors: Results of a Prospective Multicentric Study. J Clin Oncol. 2019 Jun 1;37(16):1412-1423. doi: 10.1200/JCO.18.01480. Epub 2019 Mar 15. PMID: 30875280; PMCID: PMC6544462. See more on PubMed
Summary References (17)

References to miR-371a-3p

  • Belge G, Hennig F, Dumlupinar C, Grobelny F, Junker K, Radtke A, Dieckmann KP. Graded expression of microRNA-371a-3p in tumor tissues, contralateral testes, and in serum of patients with testicular germ cell tumor. Oncotarget. 2020 Apr 21;11(16):1462-1473. doi: 10.18632/oncotarget.27565. PMID: 32363003; PMCID: PMC7185068. See more on PubMed
  • Dieckmann KP, Radtke A, Geczi L, Matthies C, Anheuser P, Eckardt U, Sommer J, Zengerling F, Trenti E, Pichler R, Belz H, Zastrow S, Winter A, Melchior S, Hammel J, Kranz J, Bolten M, Krege S, Haben B, Loidl W, Ruf CG, Heinzelbecker J, Heidenreich A, Cremers JF, Oing C, Hermanns T, Fankhauser CD, Gillessen S, Reichegger H, Cathomas R, Pichler M, Hentrich M, Eredics K, Lorch A, Wülfing C, Peine S, Wosniok W, Bokemeyer C, Belge G. Serum Levels of MicroRNA-371a-3p (M371 Test) as a New Biomarker of Testicular Germ Cell Tumors: Results of a Prospective Multicentric Study. J Clin Oncol. 2019 Jun 1;37(16):1412-1423. doi: 10.1200/JCO.18.01480. Epub 2019 Mar 15. PMID: 30875280; PMCID: PMC6544462. See more on PubMed
  • Dieckmann KP, Radtke A, Spiekermann M, Balks T, Matthies C, Becker P, Ruf C, Oing C, Oechsle K, Bokemeyer C, Hammel J, Melchior S, Wosniok W, Belge G. Serum Levels of MicroRNA miR-371a-3p: A Sensitive and Specific New Biomarker for Germ Cell Tumours. Eur Urol. 2017 Feb;71(2):213-220. doi: 10.1016/j.eururo.2016.07.029. Epub 2016 Aug 2. Erratum in: Eur Urol. 2017 May;71(5):e161. PMID: 27495845. See more on PubMed
  • Elbehidy RM, Youssef DM, El-Shal AS, Shalaby SM, Sherbiny HS, Sherief LM, Akeel NE. MicroRNA-21 as a novel biomarker in diagnosis and response to therapy in asthmatic children. Mol Immunol. 2016 Mar;71:107-114. doi: 10.1016/j.molimm.2015.12.015. Epub 2016 Feb 11. PMID: 26874829. See more on PubMed
  • Leão R, van Agthoven T, Figueiredo A, Jewett MAS, Fadaak K, Sweet J, Ahmad AE, Anson-Cartwright L, Chung P, Hansen A, Warde P, Castelo-Branco P, O'Malley M, Bedard PL, Looijenga LHJ, Hamilton RJ. Serum miRNA Predicts Viable Disease after Chemotherapy in Patients with Testicular Nonseminoma Germ Cell Tumor. J Urol. 2018 Jul;200(1):126-135. doi: 10.1016/j.juro.2018.02.068. Epub 2018 Feb 21. PMID: 29474847. See more on PubMed
  • Mego M, van Agthoven T, Gronesova P, Chovanec M, Miskovska V, Mardiak J, Looijenga LHJ. Clinical utility of plasma miR-371a-3p in germ cell tumors. J Cell Mol Med. 2019 Feb;23(2):1128-1136. doi: 10.1111/jcmm.14013. Epub 2018 Dec 7. PMID: 30536846; PMCID: PMC6349199. See more on PubMed
  • Migita K, Komori A, Kozuru H, Jiuchi Y, Nakamura M, Yasunami M, Furukawa H, Abiru S, Yamasaki K, Nagaoka S, Hashimoto S, Bekki S, Kamitsukasa H, Nakamura Y, Ohta H, Shimada M, Takahashi H, Mita E, Hijioka T, Yamashita H, Kouno H, Nakamuta M, Ario K, Muro T, Sakai H, Sugi K, Nishimura H, Yoshizawa K, Sato T, Naganuma A, Komatsu T, Oohara Y, Makita F, Tomizawa M, Yatsuhashi H. Circulating microRNA Profiles in Patients with Type-1 Autoimmune Hepatitis. PLoS One. 2015 Nov 17;10(11):e0136908. doi: 10.1371/journal.pone.0136908. PMID: 26575387; PMCID: PMC4648542. See more on PubMed
  • Nappi L, Thi M, Lum A, Huntsman D, Eigl BJ, Martin C, O'Neil B, Maughan BL, Chi K, So A, Black PC, Gleave M, Wyatt AW, Lavoie JM, Khalaf D, Bell R, Daneshmand S, Hamilton RJ, Leao RRN, Nichols C, Kollmannsberger C. Developing a Highly Specific Biomarker for Germ Cell Malignancies: Plasma miR371 Expression Across the Germ Cell Malignancy Spectrum. J Clin Oncol. 2019 Nov 20;37(33):3090-3098. doi: 10.1200/JCO.18.02057. Epub 2019 Sep 25. PMID: 31553692; PMCID: PMC7351323. See more on PubMed
  • Olivieri F, Spazzafumo L, Bonafè M, Recchioni R, Prattichizzo F, Marcheselli F, Micolucci L, Mensà E, Giuliani A, Santini G, Gobbi M, Lazzarini R, Boemi M, Testa R, Antonicelli R, Procopio AD, Bonfigli AR. MiR-21-5p and miR-126a-3p levels in plasma and circulating angiogenic cells: relationship with type 2 diabetes complications. Oncotarget. 2015 Nov 3;6(34):35372-82. doi: 10.18632/oncotarget.6164. PMID: 26498351; PMCID: PMC4742111. See more on PubMed
  • Parikh VN, Park J, Nikolic I, Channick R, Yu PB, De Marco T, Hsue PY, Chan SY. Brief Report: Coordinated Modulation of Circulating miR-21 in HIV, HIV- Associated Pulmonary Arterial Hypertension, and HIV/Hepatitis C Virus Coinfection. J Acquir Immune Defic Syndr. 2015 Nov 1;70(3):236-41. doi: 10.1097/QAI.0000000000000741. PMID: 26473639; PMCID: PMC4610144. See more on PubMed
  • Qu K, Zhang X, Lin T, Liu T, Wang Z, Liu S, Zhou L, Wei J, Chang H, Li K, Wang Z, Liu C, Wu Z. Circulating miRNA-21-5p as a diagnostic biomarker for pancreatic cancer: evidence from comprehensive miRNA expression profiling analysis and clinical validation. Sci Rep. 2017 May 10;7(1):1692. doi: 10.1038/s41598-017-01904-z. PMID: 28490741; PMCID: PMC5431820. See more on PubMed
  • Radtke A, Hennig F, Ikogho R, Hammel J, Anheuser P, Wülfing C, Belge G, Dieckmann KP. The Novel Biomarker of Germ Cell Tumours, Micro-RNA-371a-3p, Has a Very Rapid Decay in Patients with Clinical Stage 1. Urol Int. 2018;100(4):470-475. doi: 10.1159/000488771. Epub 2018 Apr 26. PMID: 29698973; PMCID: PMC6039090. See more on PubMed
  • Rosas Plaza X, van Agthoven T, Meijer C, van Vugt MATM, de Jong S, Gietema JA, Looijenga LHJ. miR-371a-3p, miR-373-3p and miR-367-3p as Serum Biomarkers in Metastatic Testicular Germ Cell Cancers Before, During and After Chemotherapy. Cells. 2019 Oct 8;8(10):1221. doi: 10.3390/cells8101221. PMID: 31597402; PMCID: PMC6830325. See more on PubMed
  • Terbuch A, Adiprasito JB, Stiegelbauer V, Seles M, Klec C, Pichler GP, Resel M, Posch F, Lembeck AL, Stöger H, Szkandera J, Pummer K, Bauernhofer T, Hutterer GC, Gerger A, Stotz M, Pichler M. MiR-371a-3p Serum Levels Are Increased in Recurrence of Testicular Germ Cell Tumor Patients. Int J Mol Sci. 2018 Oct 12;19(10):3130. doi: 10.3390/ijms19103130. PMID: 30321995; PMCID: PMC6213366. See more on PubMed
  • van Agthoven T, Looijenga LHJ. Accurate primary germ cell cancer diagnosis using serum based microRNA detection (ampTSmiR test). Oncotarget. 2016 Jul 27;8(35):58037-58049. doi: 10.18632/oncotarget.10867. PMID: 28938535; PMCID: PMC5601631. See more on PubMed
  • Wang J, Zhu Y, Jin F, Tang L, He Z, He Z. Differential expression of circulating microRNAs in blood and haematoma samples from patients with intracerebral haemorrhage. J Int Med Res. 2016 Jun;44(3):419-32. doi: 10.1177/0300060516630852. Epub 2016 Mar 28. PMID: 27020596; PMCID: PMC5536709. See more on PubMed
  • Zhang Y, Liu YJ, Liu T, Zhang H, Yang SJ. Plasma microRNA-21 is a potential diagnostic biomarker of acute myocardial infarction. Eur Rev Med Pharmacol Sci. 2016;20(2):323-9. PMID: 26875904. See more on PubMed
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