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Manufactured by BioVendor

MANF Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Mesencephalic Astrocyte-derived Neurotrophic Factor, ARMET, Arginine-rich protein (ARP)
  • Species:Human
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Cat. No. Size Price


RBG10209005 5 µg
RBG10209025 25 µg
RBG10209100 100 μg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote the survival, growth and function of dopamine-specific neurons. MANF and its structural homolog CDNF each contain a N-terminal, saposin-like, lipid-binding domain, and a carboxyl-terminal domain that is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA-induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson's di­sease. Recombinant Human MANF is an 18.1 kDa protein consisting of 158 amino acids, including 8 cysteine residues.

Amino Acid Sequence

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Source

E. coli

Purity

98%

Biological Activity

Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED 50 for this effect is 15–25 µg/ml.

Endotoxin

Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.

Storage/Expiration

 –20°C

Summary

Research topic

Neural tissue markers

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