MIP-1γ is a CC chemokine found in murine blood and a wide variety of murine tissues, with no known human homolog. MIP-1γ signals through the CCR1 receptor. MIP-1γ chemoattracts neutrophils, and also inhibits colony formation of bone marrow myeloid immature progenitors. MIP-1γ contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines. Recombinant Murine MIP-1γ is an 11.6 kDa protein containing 101 amino acid residues.
Amino Acid Sequence
Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1–10.0 ng/ml.
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. This solution can be stored at 2–8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.