MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells. Human MIP-3β is active on murine cells. Recombinant Human MIP-3β is an 8.8 kDa protein containing 77 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Amino Acid Sequence
Determined by its ability to chemoattract human T cells using a concentration of 10.0–50.0 ng/ml.
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. This solution can be stored at 2–8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.