MIP-3α is a CC chemokine, expressed in the liver, lymph nodes, appendix, PBL and lungs that can signal through the CCR6 receptor. MIP-3α is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells, and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant Murine MIP-3α is a 7.9 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Amino Acid Sequence
Determined by its ability to chemoattract murine CCR6 transfected HEK/293 cells using a concentration range of 0.1–10.0 ng/ml
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. This solution can be stored at 2–8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.