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You are here:   Home Products and Services microRNA & sncRNAs miRNA/sncRNA Quantification assays miRNA-RT-qPCR - MiRXES miRNA qPCR Assay

miRNA qPCR Assay

 

  • 610 high confidence and efficient human microRNA Assays
  • 241 high confidence and efficient mouse microRNA Assays
  • 3,381 high confidence and efficient microRNA assays from other species

 

 It may cover selected species. Ask about other species. 

The possibility of multiplexing

RT multiplex up to 10 targets

Ensure that the similarity at both 5' and 3' end are not more than 5 out of 8 base pairs.

Unparalleled Specificity

The combination of miRNA specific RT primer and nested qPCR primer pairs enables assays to efficiently discriminate highly homologous miRNA family members with single nucleotide difference.

 

10776
Assay specificity

Superior Sensitivity

The unique three primer design of the ID3EAL™ miRNA qPCR Assays yields class leading sensitivity compared to other miRNA detection systems. These assays are shown to have more consistent performance over miRNAs with varying sequences especially those with high AT content

 

10775
Sensitivity Compared


Greater Reproducibility

MiRXES ID3EAL™ miRNA qPCR assays yielded highly reproducible results in profiling more than 200 miRNAs from 30 cancer sera over a year in two independent laboratories (R2> 0.95). The ease of use enables even first time users to generate consistent technical and biological replicates. 

 

10777
Assay Reproducibility


cDNA Synthesis System

CAT. NO. Name DESCRIPTION REACTIONS
1103101 ID3EAL cDNA Synthesis System (20) For 20 x 20 μl cDNA reactions
1 x 20 μl of ID3EAL Reverse Transcriptase (20x)
1 x 100 μl of ID3EAL miRNA RT Buffer (4x)		 20
1103103 ID3EAL cDNA Synthesis System (60) For 60 x 20 µl cDNA reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
Lead time 7-14 days		 60

Individual miRNA RT Primer 1-plex

CAT. NO. Name DESCRIPTION REACTIONS
1103111 ID3EAL Individual miRNA RT Primer 1-plex (20) 20 μl of ID3EAL miRNA RT Primer (20x) 20
1103113 ID3EAL Individual miRNA RT Primer 1-plex (60) 60 μl of ID3EAL miRNA RT Primer (20x) 60
1103114 ID3EAL Individual miRNA RT Primer 1-plex (100) 100 μl of ID3EAL miRNA RT Primer (20x) 100


Individual miRNA qPCR Assay

CAT. NO. Name DESCRIPTION REACTIONS
1104101 ID3EAL Individual miRNA qPCR Assay (100) 200 μl of ID3EAL miRNA qPCR Assay (10x) 100
1104103 ID3EAL Individual miRNA qPCR Assay (500) 1000 μl of ID3EAL miRNA qPCR Assay (10x) 500

miRNA qPCR Master Mix

CAT. NO. Name DESCRIPTION REACTIONS
1104202 ID3EAL miRNA qPCR Master Mix (200) 2 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
200 x 20 μl reactions		 200
1104204 ID3EAL miRNA qPCR Master Mix (800) 8 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
800 x 20 μl reactions		 800
1104205 ID3EAL miRNA qPCR Master Mix (1200) 12 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
1200 x 20 μl reactions		 1200
1104206 ID3EAL miRNA qPCR Master Mix (2400) 24 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
2400 x 20 μl reactions		 2400
1104212 ID3EAL miRNA qPCR Master Mix - HR (200) 2 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
200 x 20 μl reactions
For High-Rox qPCR instruments		 200
1104214 ID3EAL miRNA qPCR Master Mix - HR (800) 8 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
800 x 20 μl reactions
For High-Rox qPCR instruments		 800
1104215 ID3EAL miRNA qPCR Master Mix - HR (1200) 12 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
1200 x 20 μl reactions
For High- Rox qPCR instruments		 1200
1104216 ID3EAL miRNA qPCR Master Mix - HR (2400) 24 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
2400 x 20 μl reactions
For High- Rox qPCR instruments		 2400



Product References to the MiRXES miRNA Assays

 

  • Ho YK, Xu WT, Too HP. Direct quantification of mRNA and miRNA from cell lysates using reverse transcription real time PCR: a multidimensional analysis of the performance of reagents and workflows. PLoS One. 2013 Sep 5;8(9):e72463. doi: 10.1371/journal.pone.0072463. eCollection 2013. PubMed PMID: 24039771; PubMed Central PMCID: PMC3764000. See more on PubMed 
  • Choi H, Koh HWL, Zhou L, Cheng H, Loh TP, Parvaresh Rizi E, Toh SA, Ronnett GV, Huang BE, Khoo CM. Plasma Protein and MicroRNA Biomarkers of Insulin Resistance: A Network-Based Integrative -Omics Analysis. Front Physiol. 2019 Apr 5;10:379. doi: 10.3389/fphys.2019.00379. eCollection 2019. PubMed PMID: 31024340; PubMed Central PMCID: PMC6460474. See more on PubMed 
  • Kamińska K, Nalejska E, Kubiak M, Wojtysiak J, Żołna Ł, Kowalewski J, Lewandowska MA. Prognostic and Predictive Epigenetic Biomarkers in Oncology. Mol Diagn Ther. 2019 Feb;23(1):83-95. doi: 10.1007/s40291-018-0371-7. Review. PubMed PMID: 30523565; PubMed Central PMCID: PMC6394434. See more on PubMed 
  • Davis-Vogel C, Ortiz A, Procyk L, Robeson J, Kassa A, Wang Y, Huang E, Walker C, Sethi A, Nelson ME, Sashital DG. Knockdown of RNA interference pathway genes impacts the fitness of western corn rootworm. Sci Rep. 2018 May 18;8(1):7858. doi: 10.1038/s41598-018-26129-6. PubMed PMID: 29777111; PubMed Central PMCID: PMC5959937. See more on PubMed 
  • Liu J, Xiao Y, Wu X, Jiang L, Yang S, Ding Z, Fang Z, Hua H, Kirby MS, Shou J. A circulating microRNA signature as noninvasive diagnostic and prognostic biomarkers for nonalcoholic steatohepatitis. BMC Genomics. 2018 Mar 9;19(1):188. doi: 10.1186/s12864-018-4575-3. PubMed PMID: 29523084; PubMed Central PMCID: PMC5845150. See more on PubMed  
  • Neo SH, Chung KY, Quek JM, Too HP. Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix. Sci Rep. 2017 Nov 30;7(1):16686. doi: 10.1038/s41598-017-16960-8. PubMed PMID: 29192155; PubMed Central PMCID: PMC5709463. See more on PubMed 
  • Saw WY, Tantoso E, Begum H, Zhou L, Zou R, He C, Chan SL, Tan LW, Wong LP, Xu W, Moong DKN, Lim Y, Li B, Pillai NE, Peterson TA, Bielawny T, Meikle PJ, Mundra PA, Lim WY, Luo M, Chia KS, Ong RT, Brunham LR, Khor CC, Too HP, Soong R, Wenk MR, Little P, Teo YY. Establishing multiple omics baselines for three Southeast Asian populations in the Singapore Integrative Omics Study. Nat Commun. 2017 Sep 21;8(1):653. doi: 10.1038/s41467-017-00413-x. PubMed PMID: 28935855; PubMed Central PMCID: PMC5608948. See more on PubMed 
  • Tan K, Leong SM, Kee Z, Caramat PV, Teo J, Blanco MVM, Koay ESC, Cheong WK, Soh TI, Yong WP, Pang A. Longitudinal monitoring reveals dynamic changes in circulating tumor cells (CTCs) and CTC-associated miRNAs in response to chemotherapy in metastatic colorectal cancer patients. Cancer Lett. 2018 Jun 1;423:1-8. doi: 10.1016/j.canlet.2018.02.039. Epub 2018 Mar 5. PubMed PMID: 29518480. See more on PubMed

 

Product References to the MiRXES ID3EAL miRNA qPCR Master Mix

 

  • Davis-Vogel C, Ortiz A, Procyk L, Robeson J, Kassa A, Wang Y, Huang E, Walker C, Sethi A, Nelson ME, Sashital DG. Knockdown of RNA interference pathway genes impacts the fitness of western corn rootworm. Sci Rep. 2018 May 18;8(1):7858. doi: 10.1038/s41598-018-26129-6. PubMed PMID: 29777111; PubMed Central PMCID: PMC5959937. See more on PubMed 

 

 

microRNA IN A NEW LIGHT!

microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

 

ID3EAL Biofluid miRNA Panel

ID3EAL Biofluid miRNA Panel

MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.

11029

Separately, the ID3EAL Biofluid Panel was used to illustrate distinct miRNA profiles in normal and gastric cancer patient serum.
11030
Format of Panel

 

11051 

Biofluid miRNA knowledge panel

 

CAT. NO. Name DESCRIPTION SAMPLES
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates		 6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates		 12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates		 24
1105252 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates		 6
1105253 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates		 12
1105254 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates		 24

 

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Biofluid miRNA Panel Analysis Template