ID3EAL miRNATM qPCR Assay
Re-defining miRNA Quantification with Sensitivity, Specificity and SpeedMiRXES assays are designed in silico using proprietary 30-parameter thermodynamics based algorithms and are extensively validated using both synthetic miRNAs and RNA from biological samples. Users can select from a growing library of validated human, mouse, rat, and viral miRNA assays. For non-catalog miRNAs, MiRXES offers customized assay design and validation services.
The unique three primer design of the ID3EAL™ miRNA qPCR Assays yields class leading sensitivity compared to other miRNA qPCR detection systems.
MiRXES ID3EAL™ miRNA qPCR assays provides highly reproducible results and enables even first time users to generate consistent technical and biological replicates.
|Increased sensitivity||Optimized RT-qPCR primers and reagents to drive efficient target amplification from limiting amounts (≥1pg) of input RNA sample.|
|Improved Specificity||No universal primers. Every assay utilizes three miRNA specific primers to discriminate single nucleotide differences.|
|Speedy Detection||RNA to Ct in less than 2 hours for faster turnaround and improved throughput.|
|Reliable Data||Assays optimized by MIRXES’ proprietary algorithm and wet-lab validated with synthetic miRNA templates and RNA from biological samples.|
|Convenience||Complete kit to minimize set-up time. Compatible with all major qPCR instruments.|
|Unique RT Primer||Conformational restricted miRNA specific RT primer efficiently hybridizes to mature but not precursor form of target miRNA.|
|Specific Real-Time PCR Primers||miRNA specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification of amplicon.|
|Tailored RT-qPCR Reagents||Optimized RT and qPCR master mixes enhance signal to noise ratio.|
These products are only available in these countries: Austria, Canada, Czech Republic, Germany, Ireland, Mexico, Slovakia, Switzerland, United Kingdom, United States