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You are here:   Home Products and Services microRNA & sncRNAs miRNA/sncRNA Quantification assays miRNA-RT-qPCR - MiRXES miRNA-RT-qPCR

miRNA-RT-qPCR

Re-writing the rules with microRNA diagnostics

MiRXES assays are designed in silico using proprietary 30-parameter thermodynamics based algorithms and are extensively validated using both synthetic miRNAs and RNA from biological samples. 

Users can select from a growing library of validated human, mouse, rat, and viral miRNA assays.



 

Technology

Technology

Re-defining miRNA Quantification with Sensitivity, Specificity and Speed

assay principle


The unique three primer design of the ID3EAL™ miRNA qPCR Assays yields class leading sensitivity compared to other miRNA qPCR detection systems.
 
MiRXES ID3EAL™ miRNA qPCR assays provides highly reproducible results and enables even first time users to generate consistent technical and biological replicates.

KEY BENEFITS
Increased sensitivity Optimized RT-qPCR primers and reagents to drive efficient target amplification from limiting amounts (≥1pg) of input RNA sample.
Improved Specificity No universal primers. Every assay utilizes three miRNA specific primers to discriminate single nucleotide differences.
Speedy Detection RNA to Ct in less than 2 hours for faster turnaround and improved throughput.
Reliable Data Assays optimized by MIRXES’ proprietary algorithm and wet-lab validated with synthetic miRNA templates and RNA from biological samples.
Convenience Complete kit to minimize set-up time. Compatible with all major qPCR instruments.

 


Unique Features
Unique RT Primer Conformational restricted miRNA specific RT primer efficiently hybridizes to mature but not precursor form of target miRNA.
Specific Real-Time PCR Primers miRNA specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification of amplicon.
Tailored RT-qPCR Reagents Optimized RT and qPCR master mixes enhance signal to noise ratio.


These products are only available in these countries: Austria, Canada, Czech Republic, Denmark, Finland, France, Germany, Ireland, Mexico, Norway, Slovakia, Sweden, United Kingdom, United States

miRNA qPCR Starter Kit

miRNA qPCR Starter Kit

To address the cluster of researchers who is not convinced on our technology, the ID3EAL miRNA qPCR Starter Kit will cater to their needs.

Researchers will be able to select from the pool of validated catalogue ID3EAL miRNA qPCR Assay. Limited to human assays only.


ID3EAL miRNA Starter qPCR Kit

MiRXES ID3EAL miRNA qPCR Starter Kit provides the perfect solution for research groups generating initial microRNA data. It contains all reagents required to perform 20 cDNA reactions and 200 PCR reactions. The MiRXES ID3EAL miRNA qPCR Starter Kit allows user to pick two validated primer sets of choice.

MiRXES ID3EAL Individual miRNA qPCR Assay have been optimized to provide accurate, single-plex and multi-plex microRNA detection on most real-time PCR cyclers.

Wet-lab validated assays in accordance with the MIQE guidelines.


High-performance proprietary qPCR master mix specifically designed for the MiRXES ID3EAL microRNA PCR system.


MiRXES’ patented miRNA RT-qPCR technology delivers results from RNA samples in just 2 hours.
10773


CAT. NO. Name DESCRIPTION REACTIONS
1104301 ID3EAL miRNA qPCR Starter Kit,
2 Targets (20)		 For 2 x targets
20 cDNA and 100 qPCR reactions
1 x 10 μl of ID3EAL Reverse Transcriptase (20x)
1 x 100 μl of ID3EAL miRNA RT Buffer (4x)
2 x 10 μl of ID3EAL miRNA RT Primers (20x)
1 ml of ID3EAL miRNA qPCR Master Mix
(2x),2 x 200 μl of ID3EAL miRNA qPCR Assay (10x)
Limited to human assays only		 20
1104371 ID3EAL miRNA qPCR Starter Kit,
2 Targets -Hi-ROX (20)		 For 2 x targets
20 cDNA and 100 qPCR reactions
1 x 20 µl of ID3EAL Reverse Transcriptase (20x)
1 x 100 µl of ID3EAL miRNA RT Buffer (4x)
2 x 20 µl of ID3EAL miRNA RT Primers (20x)
1 ml of ID3EAL miRNA qPCR Master Mix (2x)
2 x 200 µl of ID3EAL miRNA qPCR Assay (10x)
Limited to human assays only
For High-ROX qPCR instruments
Lead time 7-21 days		 20


Order one of our Starter Kits at sales@biovendor.com.

 

miRNA qPCR Assay

miRNA qPCR Assay

 

  • 610 high confidence and efficient human microRNA Assays
  • 241 high confidence and efficient mouse microRNA Assays
  • 3,381 high confidence and efficient microRNA assays from other species

 

 It may cover selected species. Ask about other species. 

The possibility of multiplexing

RT multiplex up to 10 targets

Ensure that the similarity at both 5' and 3' end are not more than 5 out of 8 base pairs.

Unparalleled Specificity

The combination of miRNA specific RT primer and nested qPCR primer pairs enables assays to efficiently discriminate highly homologous miRNA family members with single nucleotide difference.

 

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Assay specificity

Superior Sensitivity

The unique three primer design of the ID3EAL™ miRNA qPCR Assays yields class leading sensitivity compared to other miRNA detection systems. These assays are shown to have more consistent performance over miRNAs with varying sequences especially those with high AT content

 

10775
Sensitivity Compared


Greater Reproducibility

MiRXES ID3EAL™ miRNA qPCR assays yielded highly reproducible results in profiling more than 200 miRNAs from 30 cancer sera over a year in two independent laboratories (R2> 0.95). The ease of use enables even first time users to generate consistent technical and biological replicates. 

 

10777
Assay Reproducibility


cDNA Synthesis System

CAT. NO. Name DESCRIPTION REACTIONS
1103101 ID3EAL cDNA Synthesis System (20) For 20 x 20 μl cDNA reactions
1 x 20 μl of ID3EAL Reverse Transcriptase (20x)
1 x 100 μl of ID3EAL miRNA RT Buffer (4x)		 20
1103103 ID3EAL cDNA Synthesis System (60) For 60 x 20 µl cDNA reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
Lead time 7-14 days		 60

Individual miRNA RT Primer 1-plex

CAT. NO. Name DESCRIPTION REACTIONS
1103111 ID3EAL Individual miRNA RT Primer 1-plex (20) 20 μl of ID3EAL miRNA RT Primer (20x) 20
1103113 ID3EAL Individual miRNA RT Primer 1-plex (60) 60 μl of ID3EAL miRNA RT Primer (20x) 60
1103114 ID3EAL Individual miRNA RT Primer 1-plex (100) 100 μl of ID3EAL miRNA RT Primer (20x) 100


Individual miRNA qPCR Assay

CAT. NO. Name DESCRIPTION REACTIONS
1104101 ID3EAL Individual miRNA qPCR Assay (100) 200 μl of ID3EAL miRNA qPCR Assay (10x) 100
1104103 ID3EAL Individual miRNA qPCR Assay (500) 1000 μl of ID3EAL miRNA qPCR Assay (10x) 500

miRNA qPCR Master Mix

CAT. NO. Name DESCRIPTION REACTIONS
1104202 ID3EAL miRNA qPCR Master Mix (200) 2 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
200 x 20 μl reactions		 200
1104204 ID3EAL miRNA qPCR Master Mix (800) 8 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
800 x 20 μl reactions		 800
1104205 ID3EAL miRNA qPCR Master Mix (1200) 12 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
1200 x 20 μl reactions		 1200
1104206 ID3EAL miRNA qPCR Master Mix (2400) 24 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
2400 x 20 μl reactions		 2400
1104212 ID3EAL miRNA qPCR Master Mix - HR (200) 2 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
200 x 20 μl reactions
For High-Rox qPCR instruments		 200
1104214 ID3EAL miRNA qPCR Master Mix - HR (800) 8 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
800 x 20 μl reactions
For High-Rox qPCR instruments		 800
1104215 ID3EAL miRNA qPCR Master Mix - HR (1200) 12 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
1200 x 20 μl reactions
For High- Rox qPCR instruments		 1200
1104216 ID3EAL miRNA qPCR Master Mix - HR (2400) 24 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
2400 x 20 μl reactions
For High- Rox qPCR instruments		 2400



Product References to the MiRXES miRNA Assays

 

  • Ho YK, Xu WT, Too HP. Direct quantification of mRNA and miRNA from cell lysates using reverse transcription real time PCR: a multidimensional analysis of the performance of reagents and workflows. PLoS One. 2013 Sep 5;8(9):e72463. doi: 10.1371/journal.pone.0072463. eCollection 2013. PubMed PMID: 24039771; PubMed Central PMCID: PMC3764000. See more on PubMed 
  • Choi H, Koh HWL, Zhou L, Cheng H, Loh TP, Parvaresh Rizi E, Toh SA, Ronnett GV, Huang BE, Khoo CM. Plasma Protein and MicroRNA Biomarkers of Insulin Resistance: A Network-Based Integrative -Omics Analysis. Front Physiol. 2019 Apr 5;10:379. doi: 10.3389/fphys.2019.00379. eCollection 2019. PubMed PMID: 31024340; PubMed Central PMCID: PMC6460474. See more on PubMed 
  • Kamińska K, Nalejska E, Kubiak M, Wojtysiak J, Żołna Ł, Kowalewski J, Lewandowska MA. Prognostic and Predictive Epigenetic Biomarkers in Oncology. Mol Diagn Ther. 2019 Feb;23(1):83-95. doi: 10.1007/s40291-018-0371-7. Review. PubMed PMID: 30523565; PubMed Central PMCID: PMC6394434. See more on PubMed 
  • Davis-Vogel C, Ortiz A, Procyk L, Robeson J, Kassa A, Wang Y, Huang E, Walker C, Sethi A, Nelson ME, Sashital DG. Knockdown of RNA interference pathway genes impacts the fitness of western corn rootworm. Sci Rep. 2018 May 18;8(1):7858. doi: 10.1038/s41598-018-26129-6. PubMed PMID: 29777111; PubMed Central PMCID: PMC5959937. See more on PubMed 
  • Liu J, Xiao Y, Wu X, Jiang L, Yang S, Ding Z, Fang Z, Hua H, Kirby MS, Shou J. A circulating microRNA signature as noninvasive diagnostic and prognostic biomarkers for nonalcoholic steatohepatitis. BMC Genomics. 2018 Mar 9;19(1):188. doi: 10.1186/s12864-018-4575-3. PubMed PMID: 29523084; PubMed Central PMCID: PMC5845150. See more on PubMed  
  • Neo SH, Chung KY, Quek JM, Too HP. Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix. Sci Rep. 2017 Nov 30;7(1):16686. doi: 10.1038/s41598-017-16960-8. PubMed PMID: 29192155; PubMed Central PMCID: PMC5709463. See more on PubMed 
  • Saw WY, Tantoso E, Begum H, Zhou L, Zou R, He C, Chan SL, Tan LW, Wong LP, Xu W, Moong DKN, Lim Y, Li B, Pillai NE, Peterson TA, Bielawny T, Meikle PJ, Mundra PA, Lim WY, Luo M, Chia KS, Ong RT, Brunham LR, Khor CC, Too HP, Soong R, Wenk MR, Little P, Teo YY. Establishing multiple omics baselines for three Southeast Asian populations in the Singapore Integrative Omics Study. Nat Commun. 2017 Sep 21;8(1):653. doi: 10.1038/s41467-017-00413-x. PubMed PMID: 28935855; PubMed Central PMCID: PMC5608948. See more on PubMed 
  • Tan K, Leong SM, Kee Z, Caramat PV, Teo J, Blanco MVM, Koay ESC, Cheong WK, Soh TI, Yong WP, Pang A. Longitudinal monitoring reveals dynamic changes in circulating tumor cells (CTCs) and CTC-associated miRNAs in response to chemotherapy in metastatic colorectal cancer patients. Cancer Lett. 2018 Jun 1;423:1-8. doi: 10.1016/j.canlet.2018.02.039. Epub 2018 Mar 5. PubMed PMID: 29518480. See more on PubMed

 

Product References to the MiRXES ID3EAL miRNA qPCR Master Mix

 

  • Davis-Vogel C, Ortiz A, Procyk L, Robeson J, Kassa A, Wang Y, Huang E, Walker C, Sethi A, Nelson ME, Sashital DG. Knockdown of RNA interference pathway genes impacts the fitness of western corn rootworm. Sci Rep. 2018 May 18;8(1):7858. doi: 10.1038/s41598-018-26129-6. PubMed PMID: 29777111; PubMed Central PMCID: PMC5959937. See more on PubMed 

 

 

miRNA Spike-in RNA Kit

miRNA Spike-in RNA Kit

  • Uniquely designed small RNAs with sequences distinct from endogenous miRNAs.
  • Use to monitor and normalize experimental variations in sample RNA isolation, reverse transcription and qPCR.
  • Tested and compatible with various isolation methods:
    • Phenol/chloroform-based, phenol-free
    • Column based extraction
    • Magnetic beads
    • Precipitation methods

Spike-In RNAs to Detect Isolation Challenges

11053

Importance of Spike-in normalization

11054

Significance of normalization

11055

 


• 20 identical samples were ran

• Blue dots are data points prior spike-in normalization 

• Red dots are data points post spike-in normalization

• Note the consistent mean CT value displayed across the red dots















miRNA Spike-in RNA Kit

CAT. NO. Name DESCRIPTION REACTIONS
1102153 ID3EAL Complete Spike-in RNA Kit (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized)
1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA (lyophilized)
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x)
Lead time 7-21 days 		50
1102193 ID3EAL Complete Spike-in RNA Kit - Hi-ROX (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized)
1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x) - For High-ROX qPCR instruments
Lead time 7-21 days 		50
1102122 ID3EAL Spike-in control for Isolation (50) 1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized) for 50 cDNA reactions
Lead time 7-21 days 		50
1102112 ID3EAL Spike-in control for Reverse Transcription (50) 1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA (lyophilized) for 50 cDNA reactions
Lead time - Lead time 7-21 days 		50
1105121 ID3EAL miRNA Knowledge Panel RNA Spike-in (12) Lyophilized,
For 12 samples,
Lead time 7-21 days		 12

Product References to the MiRXES ID3EAL Spike-in control for Isolation

  • Zhu XL, Ren LF, Wang HP, Bai ZT, Zhang L, Meng WB, Zhu KX, Ding FH, Miao L, Yan J, Wang YP, Liu YQ, Zhou WC, Li X. Plasma microRNAs as potential new biomarkers for early detection of early gastric cancer. World J Gastroenterol. 2019 Apr 7;25(13):1580-1591. doi: 10.3748/wjg.v25.i13.1580. PubMed PMID: 30983818; PubMed Central PMCID: PMC6452233. See more on PubMed 

 

 


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