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miRNA-RT-qPCR

IDEAL miRNATM qPCR Assay

Re-defining miRNA Quantification with Sensitivity, Specificity and Speed

MiRXES assays are designed in silico using proprietary 30-parameter thermodynamics based algorithms and are extensively validated using both synthetic miRNAs and RNA from biological samples. Users can select from a growing library of validated human, mouse, rat, and viral miRNA assays. For non-catalog miRNAs, MiRXES offers customized assay design and validation services. 

The unique three primer design of the IDEAL miRNA qPCR Assays yields class leading sensitivity compared to other miRNA qPCR detection systems.
 
MiRXES IDEAL miRNA qPCR assays provides highly reproducible results and enables even first time users to generate consistent technical and biological replicates.

Assay principle

assay principle





KEY BENEFITS
Increased sensitivity Optimized RT-qPCR primers and reagents to drive efficient target amplification from limiting amounts (≥1pg) of input RNA sample.
Improved Specificity No universal primers. Every assay utilizes three miRNA specific primers to discriminate single nucleotide differences.
Speedy Detection RNA to Ct in less than 2 hours for faster turnaround and improved throughput.
Reliable Data Assays optimized by MIRXES’ proprietary algorithm and wet-lab validated with synthetic miRNA templates and RNA from biological samples.
Convenience Complete kit to minimize set-up time. Compatible with all major qPCR instruments.


Unique Features
Unique RT Primer Conformational restricted miRNA specific RT primer efficiently hybridizes to mature but not precursor form of target miRNA.
Specific Real-Time PCR Primers miRNA specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification of amplicon.
Tailored RT-qPCR Reagents Optimized RT and qPCR master mixes enhance signal to noise ratio.


These products are only available in these countries: Austria, Canada, Czech Republic, Germany, Ireland, Mexico, Slovakia, Switzerland, United Kingdom, United States

 

miRNA qPCR Starter Kit

miRNA qPCR Starter Kit

To address the cluster of researchers who is not convinced on our technology, the ID3EAL miRNA qPCR Starter Kit will cater to their needs.

Researchers will be able to select from the pool of validated catalogue ID3EAL miRNA qPCR Assay. Limited to human and mouse miRBase21 assays only.


ID3EAL miRNA Starter qPCR Kit

MiRXES ID3EAL miRNA qPCR Starter Kit provides the perfect solution for research groups generating initial microRNA data. It contains all reagents required to perform 20 cDNA reactions and 200 PCR reactions. The MiRXES ID3EAL miRNA qPCR Starter Kit allows user to pick two validated primer sets of choice.

MiRXES ID3EAL Individual miRNA qPCR Assay have been optimized to provide accurate, single-plex and multi-plex microRNA detection on most real-time PCR cyclers.

Wet-lab validated assays in accordance with the MIQE guidelines.


High-performance proprietary qPCR master mix specifically designed for the MiRXES ID3EAL microRNA PCR system.


MiRXES’ patented miRNA RT-qPCR technology delivers results from RNA samples in just 2 hours.

10773


CAT. NO. Name DESCRIPTION REACTIONS
1104301 ID3EAL miRNA qPCR Starter Kit,
2 Targets (20)
For 2 x targets
20 cDNA and 100 qPCR reactions
1 x 10 μl of ID3EAL Reverse Transcriptase (20x)
1 x 100 μl of ID3EAL miRNA RT Buffer (4x)
2 x 10 μl of ID3EAL miRNA RT Primers (20x)
1 ml of ID3EAL miRNA qPCR Master Mix
(2x),2 x 200 μl of ID3EAL miRNA qPCR Assay (10x)
Limited to human and mouse assays only
20
1104371 ID3EAL miRNA qPCR Starter Kit,
2 Targets -Hi-ROX (20)
For 2 x targets
20 cDNA and 100 qPCR reactions
1 x 20 µl of ID3EAL Reverse Transcriptase (20x)
1 x 100 µl of ID3EAL miRNA RT Buffer (4x)
2 x 20 µl of ID3EAL miRNA RT Primers (20x)
1 ml of ID3EAL miRNA qPCR Master Mix (2x)
2 x 200 µl of ID3EAL miRNA qPCR Assay (10x)
Limited to human and mouse assays only
For High-ROX qPCR instruments
Lead time 7-21 days
20


Order one of our Starter Kits at sales@biovendor.com.

 

miRNA qPCR Assay

miRNA qPCR Assay

It may cover selected species. Ask about other species.

Covers all human and mouse microRNA listed in the latest version of miRBase   miRBase website

For Purchase Calculation please download assay calculator here:

Assay calculator

The filled assay calculator send at sales@biovendor.com.

 

The possibility of multiplexing

RT multiplex up to 10 targets
Targets must contain at least 3 nucleotide differences in the 3´end

10801

 

Unparalleled Specificity

The combination of miRNA specific RT primer and nested qPCR primer pairs enables assays to efficiently discriminate highly homologous miRNA family members with single nucleotide difference.

 

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Assay specificity

Superior Sensitivity

The unique three primer design of the IDEAL™ miRNA qPCR Assays yields class leading sensitivity compared to other miRNA detection systems. These assays are shown to have more consistent performance over miRNAs with varying sequences especially those with high AT content

 

10775
Sensitivity Compared


Greater Reproducibility

MiRXES IDEAL™ miRNA qPCR assays yielded highly reproducible results in profiling more than 200 miRNAs from 30 cancer sera over a year in two independent laboratories (R2> 0.95). The ease of use enables even first time users to generate consistent technical and biological replicates. 

 

10777
Assay Reproducibility


cDNA Synthesis System

CAT. NO. Name DESCRIPTION REACTIONS
1103101 ID3EAL cDNA Synthesis System (20) For 20 x 20 μl cDNA reactions
1 x 20 μl of ID3EAL Reverse Transcriptase (20x)
1 x 100 μl of ID3EAL miRNA RT Buffer (4x)
20
1103103 ID3EAL cDNA Synthesis System (60) For 60 x 20 µl cDNA reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
Lead time 7-14 days
60

Individual miRNA RT Primer 1-plex

CAT. NO. Name DESCRIPTION REACTIONS
1103111 ID3EAL Individual miRNA RT Primer 1-plex (20) 20 μl of ID3EAL miRNA RT Primer (20x) 20
1103113 ID3EAL Individual miRNA RT Primer 1-plex (60) 60 μl of ID3EAL miRNA RT Primer (20x) 60
1103114 ID3EAL Individual miRNA RT Primer 1-plex (100) 100 μl of ID3EAL miRNA RT Primer (20x) 100

Customized Multiplex miRNA RT

CAT. NO. Name DESCRIPTION REACTIONS
1106114 ID3EAL Customized Multiplex miRNA RT Primers
24-plex (100)
200 µl of ID3EAL miRNA RT Primer 24-plex (10x)
Lead time 28-49 days
100
1106124 ID3EAL Customized Multiplex miRNA RT Primers
48-plex (100)
200 µl of ID3EAL miRNA RT Primer 48-plex (10x)
Lead time 28-49 days
100
1106134 ID3EAL Customized Multiplex miRNA RT Primers
96-plex (100)
200 µl of ID3EAL miRNA RT Primer 96-plex (10x)
Lead time 28-49 days
100

Individual miRNA qPCR Assay

CAT. NO. Name DESCRIPTION REACTIONS
1104101 ID3EAL Individual miRNA qPCR Assay (100) 200 μl of ID3EAL miRNA qPCR Assay (10x) 100
1104103 ID3EAL Individual miRNA qPCR Assay (500) 1000 μl of ID3EAL miRNA qPCR Assay (10x) 500

miRNA qPCR Master Mix

CAT. NO. Name DESCRIPTION REACTIONS
1104202 ID3EAL miRNA qPCR Master Mix (200) 2 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
200 x 20 μl reactions
200
1104204 ID3EAL miRNA qPCR Master Mix (800) 8 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
800 x 20 μl reactions
800
1104205 ID3EAL miRNA qPCR Master Mix (1200) 12 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
1200 x 20 μl reactions
1200
1104206 ID3EAL miRNA qPCR Master Mix (2400) 24 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
2400 x 20 μl reactions
2400
1104212 ID3EAL miRNA qPCR Master Mix - HR (200) 2 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
200 x 20 μl reactions
For High-Rox qPCR instruments
200
1104214 ID3EAL miRNA qPCR Master Mix - HR (800) 8 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
800 x 20 μl reactions
For High-Rox qPCR instruments
800
1104215 ID3EAL miRNA qPCR Master Mix - HR (1200) 12 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
1200 x 20 μl reactions
For High- Rox qPCR instruments
1200
1104216 ID3EAL miRNA qPCR Master Mix - HR (2400) 24 x 1,000 μl of ID3EAL miRNA qPCR Master Mix (2x)
2400 x 20 μl reactions
For High- Rox qPCR instruments
2400


Download assay calculator and after filling send it at sales@biovendor.com

miRNA Knowledge Panel

miRNA Knowledge Panel

 ID3EAL miRNA Knowledge Panels

Stem Cell miRNA Panel Biofluid miRNA Panel Cancer miRNA Panel MiRXES ID3EAL miRNA Knowledge Panels are ready-to-use assay panels for microRNAs relevant to specific areas of research. Each panel profiles 176 or 352 miRNAs selected through extensive survey of published literature and in-house research data. All miRNA assays have been extensively wet-lab validated using synthetic microRNA templates and human sample RNA.

 

MiRXES offers three knowledge panels for miRNA research in stem cells, biofluids and cancers. Each kit is formatted to include all necessary reagents for the RNA-to-CT workflow. Unique spike-in RNAs and inter-plate calibrators are built in to monitor and normalize technical variations from RNA isolation to qPCR. These kits are compatible with major real-time qPCR platforms and are available in various pack sizes.

 

 

10784Stem Cell miRNA Panel

192 targets (176 miRNAs)
MiRNAs are key regulators of stem cell homeostasis and differentiation. MiRNA expression patterns differ in various types of stem cells and as stem cells differentiate from a pluripotent state to specific cell lineage. The MiRXES ID3EAL Stem Cell miRNA Knowledge panel consists of 176 miRNAs which allows researchers a broadranging overview on a convenient array. See more

 

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Biofluid miRNA Panel

192 targets (176 miRNAs)
MiRNAs are selectively and actively secreted by cells as a means of inter-cellular communication. These circulating miRNAs have shown extraordinary stability in biofluids and hold great potential as novel non-invasive markers for disease diagnosis, prognosis and monitoring. The MiRXES ID3EAL Biofluid miRNA Knowledge panel consists of 176 miRNAs selected from in-house research of more than 20,000 human serum, plasma and urine specimen in healthy and pathological conditions. See more

 

10782

Cancer miRNA Panel

384 targets (352 miRNAs)
MiRNAs are intimately involved in every hallmark of cancer. Detecting miRNA dysregulations could provide insights into cancer pathogenesis, drug response and recurrence. The MiRXES ID3EAL Cancer miRNA Knowledge panel consists of 352 miRNAs strongly associated with various cancer types and regulate key oncoand tumor suppressor genes and pathways. See more


KEY BENEFITS
RNA to Results in 2 hours Using MiRXES’ patented miRNA RT-qPCR technology, the ID3EAL Knowledge Panels can produce results from RNA samples in just 2 hours.
Reliable Data Assays optimized by MIRXES’ proprietary algorithm and wet-lab validated with synthetic miRNA templates and RNA from biological samples.
Ready-to-Use Kit All reagents, including 2 spike-in RNAs and interplate PCR calibrators are provided proportionally to maximize convenience and user experience.
Automated Analysis Template An open Excel template is available for automated analysis. The template includes options for expression normalization, data visualization and data integrity.

Ask as for more information at sales@biovendor.com!

miRNA Spike-in RNA Kit

miRNA Spike-in RNA Kit

  • Uniquely designed small RNAs with sequences distinct from endogenous miRNAs.
  • Use to monitor and normalize experimental variations in sample RNA isolation, reverse transcription and qPCR.
  • Tested and compatible with various isolation methods:
    • Phenol/chloroform-based, phenol-free
    • Column based extraction
    • Magnetic beads
    • Precipitation methods

Spike-In RNAs to Detect Isolation Challenges

11053

Importance of Spike-in normalization

11054

Significance of normalization

11055

 


• 20 identical samples were ran

 Blue dots are data points prior spike-in normalization 

 Red dots are data points post spike-in normalization

 Note the consistent mean CT value displayed across the red dots















miRNA Spike-in RNA Kit

CAT. NO. Name DESCRIPTION REACTIONS
1102103 ID3EAL Spike-in RNA Kit (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x)
Lead time 7-21 days
50
1102173 ID3EAL Spike-in RNA Kit - Hi-ROX (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x) - For High-ROX qPCR instruments
Lead time 7-21 days
50
1102153 ID3EAL Complete Spike-in RNA Kit (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized)
1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA (lyophilized)
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x)
Lead time 7-21 days
50
1102193 ID3EAL Complete Spike-in RNA Kit - Hi-ROX (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized)
1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x) - For High-ROX qPCR instruments
Lead time 7-21 days
50
1102122 ID3EAL Spike-in control for Isolation (50) 1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized) for 50 cDNA reactions
Lead time 7-21 days
50
1102112 ID3EAL Spike-in control for Reverse Transcription (50) 1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA (lyophilized) for 50 cDNA reactions
Lead time - Lead time 7-21 days
50