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You are here:   Home Products and Services microRNA & sncRNAs miRNA/sncRNA Quantification assays miRNA-RT-qPCR - MiRXES miRNA Spike-in RNA Kit
miRNA Spike-in RNA Kit

miRNA Spike-in RNA Kit

  • Uniquely designed small RNAs with sequences distinct from endogenous miRNAs.
  • Use to monitor and normalize experimental variations in sample RNA isolation, reverse transcription and qPCR.
  • Tested and compatible with various isolation methods:
    • Phenol/chloroform-based, phenol-free
    • Column based extraction
    • Magnetic beads
    • Precipitation methods

Spike-In RNAs to Detect Isolation Challenges

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Importance of Spike-in normalization

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Significance of normalization

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• 20 identical samples were ran

• Blue dots are data points prior spike-in normalization 

• Red dots are data points post spike-in normalization

• Note the consistent mean CT value displayed across the red dots















miRNA Spike-in RNA Kit

CAT. NO. Name DESCRIPTION REACTIONS
1102153 ID3EAL Complete Spike-in RNA Kit (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized)
1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA (lyophilized)
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x)
Lead time 7-21 days 		50
1102193 ID3EAL Complete Spike-in RNA Kit - Hi-ROX (50) For 50 x 20 µl cDNA reactions and 200 qPCR reactions
3 x 20 µl of ID3EAL Reverse Transcriptase (20x)
3 x 100 µl of ID3EAL miRNA RT Buffer (4x)
3 x 60 µl of ID3EAL miRNA RT Primers (20x)
1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized)
1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA
2 ml of ID3EAL miRNA qPCR Master Mix (2x)
3 x 200 µl of ID3EAL miRNA qPCR Assay (10x) - For High-ROX qPCR instruments
Lead time 7-21 days 		50
1102122 ID3EAL Spike-in control for Isolation (50) 1 x 250 µl of Pre-mixed Isolation Spike-in RNA (lyophilized) for 50 cDNA reactions
Lead time 7-21 days 		50
1102112 ID3EAL Spike-in control for Reverse Transcription (50) 1 x 50 µl of Pre-mixed Reverse Transcription Spike-in RNA (lyophilized) for 50 cDNA reactions
Lead time - Lead time 7-21 days 		50
1105121 ID3EAL miRNA Knowledge Panel RNA Spike-in (12) Lyophilized,
For 12 samples,
Lead time 7-21 days		 12

Product References to the MiRXES ID3EAL Spike-in control for Isolation

  • Zhu XL, Ren LF, Wang HP, Bai ZT, Zhang L, Meng WB, Zhu KX, Ding FH, Miao L, Yan J, Wang YP, Liu YQ, Zhou WC, Li X. Plasma microRNAs as potential new biomarkers for early detection of early gastric cancer. World J Gastroenterol. 2019 Apr 7;25(13):1580-1591. doi: 10.3748/wjg.v25.i13.1580. PubMed PMID: 30983818; PubMed Central PMCID: PMC6452233. See more on PubMed 

 

 


microRNA IN A NEW LIGHT!

microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

 

ID3EAL Biofluid miRNA Panel

ID3EAL Biofluid miRNA Panel

MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.

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Separately, the ID3EAL Biofluid Panel was used to illustrate distinct miRNA profiles in normal and gastric cancer patient serum.
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Format of Panel

 

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Biofluid miRNA knowledge panel

 

CAT. NO. Name DESCRIPTION SAMPLES
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates		 6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates		 12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates		 24
1105252 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates		 6
1105253 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates		 12
1105254 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates		 24

 

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Biofluid miRNA Panel Analysis Template 

 

Find out more

miRNA Spike-in RNA KitmicroRNA IN A NEW LIGHT!ID3EAL Biofluid miRNA Panel