MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.
MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)
MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.
MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.
| CAT. NO. | Name | DESCRIPTION | SAMPLES |
| 1105152 | ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) | 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 6 x 96-well PCR plates |
6 |
| 1105153 | ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) | 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 12 x 96-well PCR plates |
12 |
| 1105154 | ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) | 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates |
24 |
| 1105252 | ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) | 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 12 x 96-well PCR plates |
6 |
| 1105253 | ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) | 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates |
12 |
| 1105254 | ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) | 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex 48 x 96-well PCR plates |
24 |
Biofluid miRNA Panel Analysis Template