Type
RT-qPCR
Description
- MiRXES Fortitude Kit 2.1, an in-vitro diagnostics (IVD) test kit, contains assays and controls for the qualitative detection of SARS-CoV-2 specific RNA, via an one-step, multiplex real-time RT-PCR reaction that involves,
Reverse transcription of purified sample RNA into cDNA
PCR amplification of two SARS-CoV-2 target sequences and the Internal Control
Simultaneous detection of the PCR amplicons by fluorescent dye labelled probes - The MiRXES Fortitude Kit 2.1 contains three unique sets of forward primers, reverse primers and probes, pre-mixed in Primers-Probes Mix 2.1 (Kit Component 6)
ORF1ab region 1 (probe with FAM fluorophore) of SARS-CoV-2 genome
ORF1ab region 2 (probe with HEX fluorophore) of SARS-CoV-2 genome
Internal Control (probe with Cy5 fluorophore) - The MiRXES Fortitude Kit 2.1 also contains three different controls
Internal Control (Kit Component 1), containing template targeted by the Internal Control assay
Positive Control 2.1 (Kit Component 2), containing SARS-CoV-2 genome regions targeted by both FAM labelled and HEX labelled SARS-CoV-2 assays
Negative Control (Kit Component 3) - Each MiRXES Fortitude Kit 2.1 contains the following 7 components in 10 vials. Each kit is sufficient for 200 reactions.
Applications
COVID-19, Nasopharyngeal swabs
Storage/Expiration
-25°C to -15°C, Protect from light