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Collagen

Soluble and Insoluble Collagen measurement Collagen is the most abundant protein found in animals. During a healthy life span the insoluble, covalent, cross-linked collagen fibers retain their biophysical functions and shapes. The collagen fibers remain isolated from most of the biochemical activities of the cells. Trauma caused by metabolic internal events, external chemical agents or physical injuries however, quickly lead to dramatic activity, resulting in rapid collagen removal followed by a wound healing response (collagen regeneration and associated remodeling). Newly formed soluble collagen production and the residual insoluble collagen fibers can be monitored using the Sircol Soluble Collagen and Sircol Insoluble Collagen Assays. Collagen proteins contain one or more domains with a triple helical structure. The three chains are described as alpha chains and should not be confused with the alpha helixes found in other proteins. The fibrillar collagens (Types I, II, III, V & XI) have most of their alpha chain structure composed of a continuous repeating tri-peptide sequence made up of glycine in every third amino acid residue [(gly-X-Y)n ]. It is to this sequence that the Sircol Dye binds. Proline is frequently an occupant in the ‘Y’ location of the tri-peptide [(gly-X-Y)n]. Many of these residues are converted, post translation, into hydroxyproline residues prior to triple helix formation and the release of the tropocollagen into the ECM. Until now, an investigator seeking to measure covalent cross-linked insoluble collagen required measurements based on free hydroxyproline content – a procedure that requires strong acid (6.0 M HCl), high temperatures (+ 95 °C) and overnight cooking (18 to 24 hrs).

34 results found in Extracellular Matrix Assays

Extracellular Matrix Assays
Product Size Price
QuickZyme Hydroxyproline Assay Kit

Type: Colorimetric assay

Discount QZBHYPRO1 96 wells (1 kit) $569,99
New QZBHYPRO2 2 x 96 wells (1 kit) $935,55
New QZBHYPRO5 5 x 96 wells (1 kit) $1663,2
 
QuickZyme Sensitive Tissue Collagen Assay Kit

Type: Colorimetric assay

Discount QZBTISCOL1 96 wells (1 kit) $701,78
New QZBTISCOL2 2 x 96 wells (1 kit) $1143,45
New QZBTISCOL5 5 x 96 wells (1 kit) $2150
 
QuickZyme Sensitive Tissue Hydroxyproline Kit

Type: Colorimetric assay

Discount QZBTISHYP1 96 wells (1 kit) $684,8
New QZBTISHYP2 2 x 96 wells (1 kit) $1120,35
New QZBTISHYP5 5 x 96 wells (1 kit) $2038,58
 
QuickZyme Soluble Collagen Assay Kit

Type: Colorimetric assay

Discount QZBCOL1-1 96 wells (1 kit) $587,03
New QZBCOL1-2 2 x 96 wells (1 kit) $964,43
New QZBCOL1-5 5 x 96 wells (1 kit) $1692,08
 
QuickZyme Total Collagen Assay Kit

Type: Colorimetric assay

Discount QZBTOTCOL1 96 wells (1 kit) $587,03
New QZBTOTCOL2 2 x 96 wells (1 kit) $964,43
New QZBTOTCOL5 5 x 96 wells (1 kit) $1740
 
QuickZyme Total Protein Assay Kit

Type: Colorimetric assay

Discount QZBTOTPROT1 96 wells (1 kit) $537,77
Discount QZBTOTPROT2 2 x 96 wells (1 kit) $837,61
New QZBTOTPROT5 5 x 96 wells (1 kit) $1472,63
 
S2000 Denatured Collagen Standard (1 mg/ml)

Type: Colorimetric assay

Discount S2010 3x 5 ml $301,45
 
S2000 Fragmentation Reagent

Type: Colorimetric assay

Discount SFRAG 3x 110 ml $351,29
 
Sircol Isolation-Concentration & Acid Neutralising Reagents (2x2x20 ml)

Type: Colorimetric assay

Discount SCONC 2x2x20 ml $301,81
 
SircolTM 2.0 Soluble Collagen Assay (96 assays)

Type: Colorimetric assay

New SIRC2 96 assays (1 kit) $590
 
Sircol™ Acid-Salt Wash

Type: Colorimetric assay

Discount SWASH 3x 20 ml $272,05
 
Sircol™ Bovine Collagen Reference Standard (0.5 mg/ml)

Type: Colorimetric assay

Discount S1010 3x 5 ml $378,02
 
Sircol™ Dye & Dye Release Reagents

Type: Colorimetric assay

Discount S1500 2x 110 ml, 2x 150 ml $712,51
 
Sircol™ Dye Reagent

Type: Colorimetric assay

S1005 3x 110 ml $696,11
 
Sircol™ Dye Release Reagent

Type: Colorimetric assay

Discount S1007 3x 150 ml $301,51
 
Sircol™ Insoluble Collagen Assay (Large Economy Kit)

Type: Colorimetric assay

Discount S6000 400 assays (1 kit) $1598,19
 
Sircol™ Insoluble Collagen Assay, Bovine Collagen (Standard Kit Size)

Type: Colorimetric assay

Discount S2000 100 assays (1 kit) $564,09
 
Sircol™ Rat Collagen Reference Standard (0.5 mg/ml)

Type: Colorimetric assay

Discount S1011 3x 5 ml $390,88
 
Sircol™ Soluble Collagen Assay (Large Economy Kit)

Type: Colorimetric assay

Discount S5000 440 assays (1 kit) $1711,96
 
Sircol™ Soluble Collagen Assay, (Standard Kit Size)

Type: Colorimetric assay

Discount S1000 110 assays (1 kit) $618,5
 
Sircol™ Soluble Collagen Assay, Rat Collagen (Standard Kit Size)

Type: Colorimetric assay

Discount S1111 110 assays (1 kit) $623,4
 
Sircol™ Soluble plus Sircol™ Insoluble Collagen Combination Pack

Type: Colorimetric assay

Discount S4000 2x 110 assays $1053,89