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Manufactured by BioVendor

NKG2D ligand 1 Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:N2DL-1, NKG2DL1, ALCAN-beta, Retinoic acid early transcript 1I, UL16-binding protein 1, ULBP1, N2DL1, RAET1I
  • Species:Human
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Cat. No. Size Price

RD172311100 0.1 mg
PubMed Product Details
Technical Data


Recombinant protein


Total 201 AA. MW: 23.6 kDa (calculated). UniProtKB Q9BZM6 (Gly26-Gly216). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence



E. coli


Purity as determined by densitometric image analysis: > 90%


< 1.0 EU/ug


Filtered (0.4 μm) and lyophilized from 0.5 mg/mL in 20mM Tris buffer, 50 mM NaCl, pH 8.6


Add 200µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.


Western blotting, ELISA


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.


This product is intended for research use only.



NKG2D ligand 1 acts as a ligand for the NKG2D receptor on the surface of NK cells. Binding of ULBPs ligands to NKG2D induces production of cytokines and chemokines. NKG2D ligand 1 is expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues. It belongs to the MHC class I family. The CMV glycoprotein UL16 interacts with NKG2D ligand 1 resulting in retention of NKG2D ligand 1 in the endoplasmic reticulum and Golgi apparatus so it does not reach the cell surface.

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