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Distributed product

NO Synthase I Human/Rat ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Species:Human, Rat
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Cat. No. Size Price

YK100 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RSCYK100R to YK100


Competitive ELISA, Immobilized antibody


Tissue extract

Sample Requirements

50 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.133–32.4 pmol/mL

Intra-assay (Within-Run)

CV = 4.0 - 5.3%

Inter-assay (Run-to-Run)

CV = 4.7 - 8.0%

Spiking Recovery

91-120 %


Research topic

Blood pressure regulation and NO metabolism, Animal studies


This human/rat NO symthase-1 EIA (NOS-1) kit is used for direct quantitative determination of the immunoreactive contents of human/rat NOS-1 presented in tissue extracts and cell culture supernatant samples. It is different from other NOS kits to date, those are NOS activity assay kits through quantitativing nitric oxide production by the enzyme. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influences by other components in samples. Human NOS-1 (998-1024) standard used in this kit is a highly purified synthetic product (purity: higher than 98%). HPLC purified biotinylated glycylglycyl-human NOS-1 (998-1024) is used as labeled antigen.

Product References (1)


  • Nakane M, Schmidt HH, Pollock JS, Förstermann U, Murad F. Cloned human brainnitric oxide synthase is highly expressed in skeletal muscle. FEBS Lett. 1993 Jan25;316(2):175-80. PubMed PMID: 7678401. See more on PubMed
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