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Distributed product

NO Synthase I Human/Rat ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Species:Human, Rat
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Cat. No. Size Price


RSCYK100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Tissue extract

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.133–32.4 pmol/mL

Intra-assay (Within-Run)

CV = 4.0 - 5.3%

Inter-assay (Run-to-Run)

CV = 4.7 - 8.0%

Spiking Recovery

91-120 %

Summary

Research topic

Blood pressure regulation and NO metabolism, Animal studies

Summary

This human/rat NO symthase-1 EIA (NOS-1) kit is used for direct quantitative determination of the immunoreactive contents of human/rat NOS-1 presented in tissue extracts and cell culture supernatant samples. It is different from other NOS kits to date, those are NOS activity assay kits through quantitativing nitric oxide production by the enzyme. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influences by other components in samples. Human NOS-1 (998-1024) standard used in this kit is a highly purified synthetic product (purity: higher than 98%). HPLC purified biotinylated glycylglycyl-human NOS-1 (998-1024) is used as labeled antigen.

References to Product

References

  • Imai T, et al (1992): Expression of brain nitric oxide synthase mRNA in various tissues and cultured cells of rat. Biomed Res 13, 371-374
  • Nakane M, et al (1993): Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle. FEBS 316, 175-180
  • Schmidt HHHW, et al (1994): Biochemistry and regulation of nitric oxide synthase, Taniguchi Symposium on Brain Sciences No.17, 3-18
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