Sandwich ELISA, Biotin-labelled antibody
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
37.5 – 2400 pg/ml
Limit of Detection
n = 8, CV = 4%
n = 6, CV = 4.9%
- It is intended for research use only
- The assay time is less than 3.5 hours
- The kit measures NT-proANP in serum and plasma (EDTA, citrate, heparin)
- Assay format is 96 wells
- Quality Controls are human serum based
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Cardiovascular disease, Sepsis
NT-proANP (N-terminal proatrial natriuretic peptide) stands for N-terminal polypeptide of ANP (atrial natriuretic peptide) precursor. ANP is one of three natriuretic peptides (atrial/ANP, B-type/BNP, C-type/CNP) characterized by similar peptide chain, degradation pathway and physiological function. Natriuretic peptides are important cardio-renal hormones because of their key role in regulation of electrolyte homeostasis and water balance as well as in regulation of blood pressure levels through diuretic, natriuretic and vasorelaxant effects.
ANP is abundantly expressed in atrial myocardial cells and stored in secretory granules as prohormone pro-ANP. During secretion N-terminal peptide of 98 amino acids (NT-proANP) is cleaved and the C-terminal peptide of 28 amino acids, ANP, which is the mature biologically active peptide, is released. ANP is rapidly removed from plasma owing to binding with its receptor, so-called clearance receptor, making its exact measurement difficult. On the other hand, NT-pro ANP, does not bind the clearance receptor, therefore, has a long half life (60–120 mins in human), and serves as an excellent marker of ANP secretion. Plasma ANP levels are increased under such pathological conditions as heart failure, myocardial infarction and hypertension. Several studies have shown that plasma ANP levels are significantly elevated in asymptomatic patients with left ventricular dysfunction. Also, plasma ANP levels increase in response to increases in right and/or left atrial pressure, while increases in BNP reflect the degree of ventricular overload.
Monitoring of NT-proANP and NT-proBNP levels can provide prognostic information for stroke and atrial fibrillation, for sepsis and for development of multiple organ dysfunction syndrome in severe trauma patients.