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Obestatin Human ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Other names:Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46
  • Species:Human
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Cat. No. Size Price

RSCYK231R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antibody


Serum, Plasma

Sample Requirements

20 μl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.412–100 ng/ml

Intra-assay (Within-Run)

CV = 3.5-9.9%

Inter-assay (Run-to-Run)

CV = 5.6-9.0%

Spiking Recovery

human serum: 101.5–113.2%
human plasma: 106.1–118.9%


Research topic

Energy metabolism and body weight regulation


Obestatin is a 23 amino acid residues peptide isolated from the rat stomach. The peptide shares the precursor with a food intake stimulating peptide, ghrelin, but possesses reducing effects on food intake, gut motility and body weight. With the use of an antiserum directed against the mouse/rat obestatin, obestatin immunoreactivity (irOBS) was detected in cells of the gastric mucosa and myenteric plexus and in Leydig cells of the testis in Sprague–Dawley rats. Double labeling of myenteric plexus with antisera against obestatin and choline acetyltransferase (ChAT) revealed that nearly all irOBS neurons were ChAT positive and vice versa. Obestatin (100nM) added to dissociated and cultured rat cerebral cortical neurons elevated cytosolic calcium concentrations [Ca+2]i in a population of cortical neurons. Intracerebroventricular admini- stration of obestatin inhibited water drinking in ad libitum fed and watered rats, and in food and water deprived animals. In addition, obestatin inhibited angiotensin II-induced water drinking in animals provided free access to water and food. Obestatin peptides had no effect on insulin sensitivity as revealed by hypoglycaemic response when co-administered with insulin, supporting a role of obestatin in regulating metabolism through changes
of appetite, but indicating no direct actions on glucose homeostasis or insulin secretion. It is supposed that in rats the effects of obestatin on food intake may be secondary to an action of the peptide to inhibit water drinking.
The obestatin concerning study for energy homeostasis and body weight regulation could be expected to have a large development in the future. The human obestatin EIA assay kit developed by our laboratory can be used for direct determination of blood obestatin level’s variations and will be a useful tool for further development of obestatin research.

References to Product


  • Gungor S, Gurates B, Aydin S, Sahin I, Kavak SB, Kumru S, Celik H, Aksoy A, Yilmaz M, Catak Z, Citil C, Baykus Y, Deniz R, Karakaya F, Ozdemir N. Ghrelins, obestatin, nesfatin-1 and leptin levels in pregnant women with and without hyperemesis gravidarum. Clin Biochem. 2013 Jun;46 (9):828-30
References to Summary

References to Obestatin

  • Dun SL, Brailoiu GC, Brailoiu E, Yang J, Chang JK, Dun NJ. Distribution and biological activity of obestatin in the rat. J Endocrinol. 2006 Nov;191 (2):481-9
  • Green BD, Irwin N, Flatt PR. Direct and indirect effects of obestatin peptides on food intake and the regulation of glucose homeostasis and insulin secretion in mice. Peptides. 2007 May;28 (5):981-7
  • Nagaraj S, Peddha MS, Manjappara UV. Fragments of obestatin as modulators of feed intake, circulating lipids, and stored fat. Biochem Biophys Res Commun. 2008 Feb 15;366 (3):731-7
  • Ren AJ, Guo ZF, Wang YK, Wang LG, Wang WZ, Lin L, Zheng X, Yuan WJ. Inhibitory effect of obestatin on glucose-induced insulin secretion in rats. Biochem Biophys Res Commun. 2008 May 9;369 (3):969-72
  • Samson WK, White MM, Price C, Ferguson AV. Obestatin acts in brain to inhibit thirst. Am J Physiol Regul Integr Comp. 2007 Jan;292 (1):R637-43
  • Scrima M, Campiglia P, Esposito C, Gomez-Monterrey I, Novellino E, D'Ursi AM. Obestatin conformational features: a strategy to unveil obestatin's biological role?. Biochem Biophys Res Commun. 2007 Nov 23;363 (3):500-5
  • Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science. 2005 Nov 11;310 (5750):996-9
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