Total 221 AA. MW: 24.4 kDa (calculated). UniProtKB acc.no. Q8IYS5–2 Isoform 2 (Asp19-Asn229). N-terminal his-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 95%
14 % SDS-PAGE separation of Human Oscar (E.coli):
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and boiled sample, 2.5 μg/lane
3. Non-reduced and non-boiled sample, 2.5 μg/lane
< 1.0 EU/μg
0,3–0,6 mg/ml in 50mM acetate buffer, pH4.0, 20%(w/v) glycerol – filtered (0.4 μm), frozen
Thaw at ambient temperature
Western blotting, ELISA
On ice. Upon receipt, store the product at the temperature recommended below.
Aliquot thawed protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Thawed protein should be used within one day to avoid forming of agragates.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.