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PDGF-BB Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Platelet-Derived Growth Factor-BB,Platelet-derived growth factor subunit B, PDGF-2, PDGF beta polypeptide, Proto-oncogene c-Sis, Becaplermin, PDGFB, PDGF2, SIS
  • Species:Human
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New RD172578100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 116 AA. MW: 13.2 kDa (calculated). UniProtKB acc. no. P01127 (Ser82-Thr190). Initial methionine and C-terminal His-tag. Homodimer. Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVTHHHHHH

Source

E. coli

Purity

Purity as determined by densitometric image analysis: > 95 %

SDS-PAGE Gel

14 % SDS-PAGE separation of Huma PDGF-BB:

  1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
  2. Reduced and boiled sample, 2.5 μg/lane
  3. Non-reduced and non-boiled sample, 2.5 μg/lane

Biological Activity

The biological activity of PDGF-BB is measured by its stimulation of proliferation of mouse fibroblasts 3T3. The ED50 for the effect is 3-5 ng/ml.

Endotoxin

< 0.1 EU/μg

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, pH 7.7.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.

Applications

Western blotting, ELISA, Cell culture and/or animal studies

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Bone and cartilage metabolism, Cardiovascular disease, Coronary artery disease, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology

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