Total 424 AA. MW: 48.4 kDa (calculated). UniProtKB acc. No. P16671 (Gly30–Asn439). N-terminal linker (2 extra AA), C-terminal linker (2 extra AA) and C-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: >90%
12 % SDS-PAGE separation of Human Platelet Glycoprotein 4:
1. MW marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and boiled sample, 2.5 μg/lane
3. Non-reduced and non-boiled sample, 2.5 μg/lane
< 1.0 EU/ug
0.3 – 0.5 mg/ml in PBS, pH = 7.4, 5 % trehalose – filtered (0.4 μm), frozen
Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA
On ice. Upon receipt, store the product at the temperature recommended below.
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.