Sandwich ELISA, HRP-labelled antibody
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box). Do not freeze.
Limit of Detection
- It is the responsibility of the user to check the compatibility of the assay with the study matrix
Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation
TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccharides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.