Sandwich ELISA, HRP-labelled antibody
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box). Do not freeze.
Limit of Detection
- It is the responsibility of the user to check the compatibility of the assay with the study matrix
Animal studies, Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation
TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccharides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.