Sandwich ELISA, Biotin-labelled antibody
Serum, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Amniotic fluid, Plasma
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
31.3 – 2000 pg/ml
Limit of Detection
n=8; CV = 5.2%
n=6; CV = 3.1%
- The total assay time is less than 3.5 hours
- The kit measures PREF-1 in human serum, plasma (EDTA, citrate, heparin), urine, cerebrospinal fluid (CSF), bronchoalveolar lavage fluid (BALF) and amniotic fluid
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Energy metabolism and body weight regulation, Oncology
Preadipocyte factor 1 (Pref-1), also called Delta-like 1 (DLK1) or Fetal antigen 1 (FA1), is a protein consisting of 385 amino acids, member of the epidermal growth factor-like (EGF-like) family of proteins. It is highly expressed in pre-adipocytes, but decreases during the process of differentiation and is absent in mature adipocytes. Pref-1 was originally identified as an inhibitor of adipocyte differentiation. Pref-1 is expressed in most embryonic and fetal tissues. Postnatally, Pref-1 expression disappears from most of these tissues, but is associated with pediatric malignancies, such as hepatoblastoma, neuroblastoma, and nephroblastoma, and some adult malignancies, such as myelodysplastic syndrome, pituitary tumors, breast, colon and prostate carcinoma. Pref-1 is found in most biological fluids, including urine, serum, amniotic fluid and cord serum. Experimental studies suggest that circulating Pref-1 levels may be also involved in the regulation of lipid and glucose metabolism and energy homeostasis. Measurement of Pref-1 within the context of human obesity reveals that serum levels increase with obesity, are negatively associated with insulin sensitivity and modulate inflammation in vitro. Pref-1 is a sensitive indicator of metabolic adaptation during weight change and changes in Pref-1 serum levels reflect changes in adipose tissue turnover.