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Manufactured by BioVendor

PREF-1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Pref-1, Protein Delta Homolog 1, Preadipocyte factor 1, DLK-1, pG2, Fetal antigen 1, FA1, Dlk1, Dlk, Pref1
  • Species:Human
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New RD191519200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Amniotic fluid, Plasma

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

31.3 – 2000 pg/ml

Limit of Detection

13 pg/ml

Intra-assay (Within-Run)

n=8; CV = 5.2%

Inter-assay (Run-to-Run)

n=6; CV = 3.1%

Spiking Recovery

101.0%

Dilutation Linearity

99.4%

Summary

Features

  • The total assay time is less than 3.5 hours
  • The kit measures PREF-1 in human serum, plasma (EDTA, citrate, heparin), urine, cerebrospinal fluid (CSF), bronchoalveolar lavage fluid (BALF) and amniotic fluid
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Energy metabolism and body weight regulation, Oncology

Summary

Preadipocyte factor 1 (Pref-1), also called Delta-like 1 (DLK1) or Fetal antigen 1 (FA1), is a protein consisting of 385 amino acids, member of the epidermal growth factor-like (EGF-like) family of proteins. It is highly expressed in pre-adipocytes, but decreases during the process of differentiation and is absent in mature adipocytes. Pref-1 was originally identified as an inhibitor of adipocyte differentiation. Pref-1 is expressed in most embryonic and fetal tissues. Postnatally, Pref-1 expression disappears from most of these tissues, but is associated with pediatric malignancies, such as hepatoblastoma, neuroblastoma, and nephroblastoma, and some adult malignancies, such as myelodysplastic syndrome, pituitary tumors, breast, colon and prostate carcinoma. Pref-1 is found in most biological fluids, including urine, serum, amniotic fluid and cord serum. Experimental studies suggest that circulating Pref-1 levels may be also involved in the regulation of lipid and glucose metabolism and energy homeostasis. Measurement of Pref-1 within the context of human obesity reveals that serum levels increase with obesity, are negatively associated with insulin sensitivity and modulate inflammation in vitro. Pref-1 is a sensitive indicator of metabolic adaptation during weight change and changes in Pref-1 serum levels reflect changes in adipose tissue turnover.

References to Summary

References to Protein Delta Homolog 1

  • Jensen CH, Krogh TN, Højrup P, Clausen PP, Skjødt K, Larsson LI, Enghild JJ, Teisner B: Protein structureof fetal antigen 1 (FA1). Eur J Biochem 1994, 225:83-92
  • Jensen CH, Erb K, Westergaard LG, Kliem A, Teisner B: Fetal antigen 1, an EGF multidomain protein in the sex hormone producing cells of the gonads and the microenvironment of germ cells. Molecul Hu Reproduc 1999, 5(10):908-913
  • Sul HS, Smas C, Mei B, Zhou L: Function of pref-1 as an inhibitor of adipocyte differentiation. 2000 Internat J Obesity, 24(4):S15-S19
  • Wang Y, Kim KA, Kim JH Sul HS: Pref-1, a preadipocyte secreted factor that inhibits adipogenesis. J Nutr 2006, 136:2953-2956
  • O´Connell J, Lynch L, Hogan A, Cawood TJ, O´Shea D: Preadipocyte factor-1 is associated with metabolic profile in severe obesity. J clin Endocrin Metab 2011, 96(4):E680-E684
  • Chacón MR, Miranda M, Jensen CH, Fernández-Real JM, Vilarrasa N, Gutiérrez C, Näf S, Gomez JM, Vendrell J: Human serum levels of fetal antigen 1 (FA1/Dlk1) increase with obesity, are negatively associated with insulin sensitivity and modulate inflammation in vitro. Int J Obesity 2008, 32:1122-1129
  • Kavalkova P, Dostalova I, Haluzikova D, Trachta P, Hanusová V, Lacinova Z, Papezova H, Domluvilova D, Zikan V, Haluzik M: Preadipocyte factor-1 concentration in patients with anorexia nervosa: the influence of partial realimentation. Physiol Res 2012, 61:153-159
  • Falix FA, Aronson DC, Lamers WH, Hiralall JK, Seppen J: DLK1, a serum marker for hepatoblastoma in young infants. Pediatr Blood Cancer 2012, 59(4):743-745
  • Mitterberger MC, Lechner S, Mattesich M, Kaiser A, Probst D, Wenger N, Pierer G, Zwerschke W: DLK1(Pref1) is a negative regulator of adipogenesis in CD105+/CD90+/CD34+/CD31-/FABP4- adipose-derived stromal cells from subcutaneous abdominal fat pats of adult women. Stem Cell Res 2012, 9:35-48
  • Andries A, Niemeier A, Stoving RK, Abdallah BM, Wolf AM, Horder K, Kassem M: Serum levels of fetal antigen 1 extreme nutritional states. ISRN Endocrinology 2012, doi:10.5402/2012/592648Andries A, Niemeier A, Stoving RK, Abdallah BM, Wolf AM, Horder K, Kassem M: Serum levels of fetal antigen 1 extreme nutritional states. ISRN Endocrinology 2012, doi:10.5402/2012/592648
  • Hudak CS, Sul HS: Pref-1, a gatekeeper of adipogenesis. Front Endocrin 2013, 4:79 Hudak CS, Sul HS: Pref-1, a gatekeeper of adipogenesis. Front Endocrin 2013, 4:79
  • Li H, Cui ML, Chen TY, Xie HY, Cui Y, Tu H, Chen FH, Ge C, Li JJ: Serum DLK1 is a potential prognostic biomarker in patients with hepatocellular carcinoma. Tumor Biol 2015, 36(11):8399-8404
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