Sandwich ELISA, Biotin-labelled antibody
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
12.5 – 800 pg/ml
Limit of Detection
n = 8; CV = 4.2 %
n = 6; CV = 7.1 %
Serum: 95.9 %
Urine: 94.8 %
Serum: 100.1 %
Urine: 94.3 %
- It is intended for research use only
- The total assay time is less than 3.5 hours
- The kit measures canine procalcitonin in serum and urine
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Immune Response, Infection and Inflammation, Sepsis, Animal studies
Procalcitonin (PCT), a polypeptide with a molecular mass of about 13 kDa, is the precursor of calcitonin. PCT is constitutively produced in the C cells of the thyroid gland and does not exhibit hormone activity. It undergoes successive cleavages to form three distinct molecules; calcitonin, katacalcin and an N-terminal fragment called aminoprocalcitonin. PCT belongs to a group of related proteins called the CAPA (CGRP – Amylin – Pro-calcitonin – Adrenomedullin) protein family . Synthesis of PCT is regulated by the CALC-I gene. Circulating levels of PCT in healthy human individuals was found to be very low (depend on source < 0.1 ng/ml or < 0.5 ng/ml).
PCT is rapidly produced and released to peripheral circulation in response to endotoxin and pro-inflammatory cytokines, such as IL-1β and TNF-α. During microbial infection, there is an increase of CALC-I gene expression which causes a release of PCT from all parenchymal tissues and differentiated cell types throughout the body, including the liver and peripheral blood mononuclear cells.
PCT is markedly elevated within 2 to 4 hours in severe forms of systemic inflammation or in bacterial infections, and the level persists until recovery. The biological half-life of PCT is 22 to 26 hours, an advantageous time point compared with CRP and other acute-phase reactants. Because up-regulation of PCT is attenuated by INF-γ, a cytokine released in response to viral infections, PCT is more specific for bacterial infections and may help to distinguish bacterial infections from viral illnesses.
It should be noted that PCT is also elevated in noninfectious conditions such as trauma, surgery, cardiogenic shock, burns, heat stroke, acute respiratory distress syndrome, infected necrosis after acute pancreatitis, and rejection after transplantation.
PCT is used as an early biomarker for the diagnosis of sepsis, severe sepsis and septic shock. PCT has also proved to be useful in guiding antibiotic therapy. This approach was mainly evaluated in patients with respiratory tract infections; however, it can also be used in critically ill patients with sepsis or severe sepsis of various origins.
Severe bacterial infections can result in marked morbidity and death in veterinary patients, with 50–70% of dogs with sepsis succumbing to their disease. Early diagnosis of infection is essential for the appropriate management of sepsis, as it allows rapid administration of antibiotics resulting in improved outcomes. Although PCT mRNA expression from nonthyroidal tissue has been demonstrated in dogs with inflammation, sepsis and SIRS, very little is known about serum PCT concentration in dogs due to the lack of a validated canine assay.