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Anti-Alpha-Galactosyl IgE Human ELISA

Other names: Anti-Alpha-Galactosyl Antibody Product of BioVendor
Product: Size:
New: RD199429200R (regulatory status: RUO) 96 wells (1 kit)
Files: Datasheet PDF (RUO)MSDS (RUO)Product description Anti-Alpha-Galactosyl IgE on pubmed

Product details


Summary

Carbohydrate moieties are frequently encountered in food and can elicit IgE responses. The recent work has identified a novel IgE antibody response to a mammalian oligosaccharide epitope, galactose-alpha-1,3-galactose (α-Gal). IgE antibodies to galactose-α-1,3-galactose are capable of eliciting serious, even fatal, reactions. Alpha-Gal is a blood group substance of nonprimate mammals, a carbohydrate commonly expressed on a large number of nonprimate mammalian proteins. Some of the patients with positive skin tests to cat allergens have IgE antibodies specific for an oligosaccharide on cat IgA. The dominant human IgE epitope on cat IgA2 is the carbohydrate α-Gal. The oligosaccharide α-Gal is also present on the F(ab′)2 part of the monoclonal antibody cetuximab used in biological therapy for cancer. The immunoglobulin E (IgE) antibody response to the carbohydrate Galα1–3Galβ1–4GlcNAc-R is associated with allergies to red meat, cetuximab, and gelatin. The relationship between the exposure to an antigen and the subsequent allergic symptoms is usually obvious. However, two different and novel forms of anaphylaxis have now been associated with IgE to α-Gal, first of them being characterized by delayed onset of symptoms: (1) Delayed-onset anaphylaxis occurs 3 to 6 hours after ingestion of mammalian food products – meat (e.g. beef, pork, or lamb), organs, gelatin or milk of the animals that carry this oligosaccharide. The characteristic features of the syndrome of delayed anaphylaxis to red meat (DARM) are late onset of urticarial (hives), anaphylactic episodes, or angioedema, together with no immediate cause, a history of pruritic tick bites, and exposure to red meat 3 to 6 hours before the onset of symptoms. (2) This epitope has, in addition, been demonstrated to be responsible for potentially life-threatening anaphylactic reactions to cetuximab in patients who have specific IgE antibodies to α-Gal; in these patients, immediate-onset anaphylaxis during first exposure to intravenous cetuximab has been described. Although IgM, IgA and IgG antibodies to α-Gal (anti-α-Gal or anti-Gal) are widely expressed in humans, IgE anti-α-Gal antibodies are not. There is now extensive evidence that the primary impulse to start production of these IgE antibodies is a tick bite or parasitic infestation. The association between tick bites, red meat allergy and IgE to alpha-Gal has been reported from four continents to date. On the other hand, anti-α-Gal IgG and IgM antibodies could contribute to opsonization or blocking of α-Gal motifs on tick proteins, avoiding or reducing development of detrimental anti-α-Gal IgE response. Current evidence suggests that screening for IgE antibodies against the α-Gal epitope may help to identify patients who are at risk of anaphylaxis, including patients who start cetuximab treatment.

Areas of investigation: Immunology, Allergology, Immune Response, Biological Therapy, Oncology

Features

  • The total assay time is less than 3.5 hours
  • The kit measures IgE anti-alpha-galactosyl antibodies in human serum
  • Assay format is 96 wells
  • Calibrator is human serum based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Immunology, Oncology


Assay format

Capture ELISA, Biotin-labelled antibody

Applications

Serum

Sample requirements

10 ul/well

Storage/Shipping

Store the complete kit at 2 8 °C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

0.04–1.28 U/ml

Limit of detection

0.01 U/ml

Intra-assay (Within-Run, n=8)

CV = 6%

Inter-assay (Run-to-Run, n=5)

CV = 6.2%

Spiking Recovery

93.6%

Dilution Linearity

98.6%


References to summary

  • Cabezas-Cruz A, Mateos-Hernandez L, Perez-Cruz M, Valdes JJ, Mera IG, Villar M, de la Fuente J. Regulation of the Immune Response to alpha-Gal and Vector-borne Diseases. Trends Parasitol. 2015 Oct;31 (10):470-6
  • Commins SP, Satinover SM, Hosen J, Mozena J, Borish L, Lewis BD, Woodfolk JA, Platts-Mills TA. Delayed anaphylaxis, angioedema, or urticaria after consumption of red meat in patients with IgE antibodies specific for galactose-alpha-1,3-galactose. J Allergy Clin Immunol. 2009 Feb;123 (2):426-33
  • Hamanova M, Chmelikova M, Nentwich I, Thon V, Lokaj J. Anti-Gal IgM, IgA and IgG natural antibodies in childhood. Immunol Lett. 2015 Mar;164 (1):40-3
  • Platts-Mills TA, Schuyler AJ, Tripathi A, Commins SP. Anaphylaxis to the carbohydrate side chain alpha-gal. Immunol Allergy Clin North Am. 2015 May;35 (2):247-60
  • Rispens T, Derksen NI, Commins SP, Platts-Mills TA, Aalberse RC. IgE production to alpha-gal is accompanied by elevated levels of specific IgG1 antibodies and low amounts of IgE to blood group B. PLoS One. 2013;8 (2):e55566
  • Steinke JW, Platts-Mills TA, Commins SP. The alpha-gal story: lessons learned from connecting the dots. J Allergy Clin Immunol. 2015 Mar;135 (3):589-96; quiz 597
  • Verresen D, De Backer W, Van Meerbeeck J, Neetens I, Van Marck E, Vermeire P. Spherocytosis and pulmonary hypertension coincidental occurrence or causal relationship?. Eur Respir J. 1991 May;4 (5):629-31
  • Woodfolk JA, Commins SP, Schuyler AJ, Erwin EA, Platts-Mills TA. Allergens, sources, particles, and molecules: Why do we make IgE responses?. Allergol Int. 2015 Oct;64 (4):295-303


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