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Mouse Interleukin-6 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-6, Interferon-beta 2, B-cell stimulatory factor 2,HGF
  • Species:Mouse
Cat. No. Size Price


RAF071R 96 wells (1 kit) $694,28
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Description

The mouse IL-6 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-6. The mouse IL-6 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Applications

Serum, Plasma-EDTA, Plasma-Citrate, Cell culture supernatant

Sample Requirements

50 µl/well

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

31.3–2000 pg/ml

Limit of Detection

6.5 pg/ml

Intra-assay (Within-Run)

CV = 5.0%

Inter-assay (Run-to-Run)

CV = 8.9%

Specificity

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-6 positive serum.There was no cross reactivity observed with mouse IL-17, IL-2, IFN-γ, TFN-α, IL-5, IL-1α, GM- SF, IL-4, IL-10.

Summary

Features

  • RUO
  • calibration range: 31.3-2 000 pg/ml
  • limit of detection 6.5 pg/ml
  • intra-assay CV = 5.0%
  • inter-assay CV = 8.9%

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Sepsis, Animal studies, COVID-19

Summary

Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells. IL-6 is involved in: − the induction of B-cell differentiation, − the induction of acute phase proteins in liver cells, − growth promotion of myeloma/plasmacytoma/hybridoma cells, − induction of IL-2 and IL-2 receptor expression, − proliferation and differentiation of T cells, − inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages, − enhancement of IL-3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor, − induction of mesangial cell growth, − induction of neural differentiation of PC 12 cells and − induction of keratinocyte growth

Summary References (7)

References to Interleukin-6

  • Cayphas S, Van Damme J, Vink A, Simpson RJ, Billiau A, Van Snick J. Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. J Immunol. 1987 Nov 1;139 (9):2965-9
  • Hirano T, Akira S, Taga T, Kishimoto T. Biological and clinical aspects of interleukin 6. Immunol Today. 1990 Dec;11 (12):443-9
  • Hirano T, Kishimoto T. Interleukin-6.Handbook of experimental pharmacology, Peptide Growth Factors and Their Receptors. Springer-Verlag. 1990;:633-665
  • Hirano T, Taga T, Nakano N, Yasukawa K, Kashiwamura S, Shimizu K, Nakajima K, Pyun KH, Kishimoto T. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci U S A. 1985 Aug;82 (16):5490-4
  • Koj A. The Role of Interleukin-6 as the Hepatocyte Stimulating Factor in the Network of Inflammatory Cytokines. Annals of the New York Academy. (1989);:557: 1–8
  • Nordan RP, Potter M. A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science. 1986 Aug 1;233 (4763):566-9
  • Ray A, Tatter SB, Santhanam U, Helfgott DC, May LT, Sehgal PB. Regulation of expression of interleukin-6. Molecular and clinical studies. Ann N Y Acad Sci. 1989;557:353-61; discussion 361-2
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