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Calmodulin-Like Protein 5 Human E. coli

Type: Recombinant
Source: E. coli Species: Human
Other names: CLSP, Calmodulin-like skin protein, CALML5 Product of BioVendor
Product: Size:
New: RD172501100 0.1 mg
Files: Datasheet PDFMSDS Calmodulin-Like Protein 5 Human on pubmed

Product details


Description

Total 155 AA. MW: 17.0 kDa (calculated). UniProtKB acc. no. Q9NZT1 (Ala2–Glu146). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MKHHHHHHAS AGELTPEEEA QYKKAFSAVD TDGNGTINAQ ELGAALKATG KNLSEAQLRK LISEVDSDGD GEISFQEFLT AAKKARAGLE DLQVAFRAFD QDGDGHITVD ELRRAMAGLG QPLPQEELDA MIREADVDQD GRVNYEEFAR MLAQE

Source

E. coli

Purity

Purity as determined by densitometric image analysis: > 95%

SDS-PAGE gel

14 % SDS-PAGE separation of Human CLSP:

  1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
  2. Reduced and boiled sample, 2.5 μg/lane
  3. Non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 1.0 EU/μg

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, 5% w/v trehalosa, pH 7.5.

Reconstitution

Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage, Stability/Shelf Life

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS-PAGE to determine purity of the protein.

LAL TEST to determine endotoxin level.

Applications

ELISA, Western blotting

Physical Appearance

This product is intended for research use only.



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