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Major Urinary Protein 1 Mouse E. coli

Type: Recombinant
Source: E. coli Species: Mouse
Other names: MUP1, MUP-1 Product of BioVendor
Product: Size:
New: RD272521100 0.1 mg
Files: Datasheet PDFMSDS Major Urinary Protein 1 on pubmed

Product details


Description

Total 172 AA. MW: 19.9 kDa (calculated). UniProtKB acc. no. P11588 (Glu19-Glu180). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MKHHHHHHAS EEASSTGRNF NVEKINGEWH TIILASDKRE KIEDNGNFRL FLEQIHVLEN SLVLKFHTVR DEECSELSMV ADKTEKAGEY SVTYDGFNTF TIPKTDYDNF LMAHLINEKD GETFQLMGLY GREPDLSSDI KERFAQLCEK HGILRENIID LSNANRCLQA RE

Source

E. coli

Purity

Purity as determined by densitometric image analysis: > 95 %

SDS-PAGE gel

14 % SDS-PAGE separation of Mouse MUP1:

  1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
  2. Reduced and boiled sample, 2.5 μg/lane
  3. Non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 1.0 EU/μg

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, 5% w/v trehalose, pH 7.5.

Reconstitution

Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage, Stability/Shelf Life

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS-PAGE to determine purity of the protein.

LAL TEST to determine endotoxin level.

Applications

ELISA, Western blotting

Note

This product is intended for research use only.



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