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Endotoxin removal

EndoTrap®, affinity chromatography for highly efficient endotoxin removal, manufactured by Hyglos GmbH.

EndoTrap® reliably removes endotoxins (lipopolysaccharides / LPS) from aqueous solutions of e.g. proteins, antibodies, vaccines, nucleic acids, buffers and various other substances with excellent sample recovery rates.

Features:

  • Highly specific and efficient LPS removal from complex mixtures
  • Quick and easy to use in column (gravity flow) or batch mode
  • Upscaling on fully automated liquid-chromatographic systems possible
  • Average sample/protein recovery > 95%
  • Broad pH tolerance and broad pI range of target proteins
  • Compatible with commonly used buffers and high salt conditions
  • Reusable at least 10 times (EndoTrap® HD)
  • Not Polymyxin B based, non toxic

The EndoTrap® product family consists of three members:

EndoTrap® blue
with a broad pH and salt tolerance, works best with calcium or magnesium compatible buffers such as TRIS, HEPES, MOPS but also PBS, if enriched freshly with 0.1 mM Ca/Mg2+.

EndoTrap® red
especially for the use with PBS based samples.

EndoTrap® HD
is a further development of EndoTrap EndoTrap® blue for challenging samples and large scale endotoxin removal in e.g biopharmaceutical production processes. For EndoTrap® HD additionally is available the EndoTrap® leakage ELISA.


EndoGrade® Endotoxin-free Ovalbumin

321000 - Ovalbumin EndoGrade 10 mg 10 mg
321001 - Ovalbumin EndoGrade 100 mg 100 mg
321002 - Ovalbumin EndoGrade 1000 mg 1000 mg

hyBeads® Streptavidin

311092 4 × 10 mg

EndoTrap® red

321053 - EndoTrap® red 1/1 1x1 ml column
321063 - EndoTrap® red 5/1 5x 1 ml column
321064 - EndoTrap® red 10 10 ml resin
321075 - EndoTrap® red 50 50 ml resin

EndoTrap®HD

800001 - EndoTrap® HD 5x Regeneration Buffer 500 ml (equals 2.5 L buffer) 500 ml
800033 - EndoTrap® Leakage ELISA 96-wells
800034 - EndoTrap® HD 10 10 ml
800035 - EndoTrap® HD 50 50 ml
800036 - EndoTrap® HD 250 250 ml
800037 - EndoTrap® HD Bulk > 1 L
800044 - EndoTrap® HD 5x Equilibration Buffer 500 ml (equals 2.5 L buffer) 500 ml
800046 - EndoTrap® HD 5x Storage 500 ml (equals 2.5 L buffer) 500 ml
800053 - EndoTrap HD 1/1 1x1 ml column
800063 - EndoTrap HD 5/1 5x 1 ml column

EndoTrap® accessory equipment

321080 - EndoTrap® red Regeneration Buffer 125 ml
321081 - EndoTrap® red Equilibration Buffer 125 ml
321082 - EndoTrap® red Low Salt Equilibration Buffer 125 ml
800003 - EndoTrap® red Regeneration Buffer, 10X 1000 ml
800006 - Disposable Polypropylene Funnel 5 pack
800007 - Empty Columns, Large 5 pack
800008 - Empty Columns, Small 5 pack


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