Sandwich ELISA, HRP-labelled antibody
Serum, Plasma (EDTA, citrate, heparin)
Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
n = 8; CV = 5.2 %
n = 6; CV = 4.1 %
- bovine Non-detectable
- cat Non-detectable
- dog Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Non-detectable
- monkey Non-detectable
- mouse Non-detectable
- pig Non-detectable
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- chicken Not tested
- human Yes
- It is intended for research use only
- The total assay time is less than 3 hours
- The kit measures total proguanylin in serum, plasma
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Others, Renal disease
Proguanylin, the 116-amino acid prohormone, is a bioactive form of human guanylin that acts on intestinal guanylate cyclase, thereby regulating intestinal fluid and elektrolyte transport through the second messenger, cyclic GMP (cGMP). The cGMP increase inhibits salt absorption and stimulates chloride secretion into the gut. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to diarrhea and dehydratation characteristic of enterotoxin activity.
Proguanylin is found in circulation and plays an endocrine role by regulating the function of tissues such as the kidney and liver. Proguanylin is a significant marker in renal insufficiency. Plasma levels of proguanylin increase in patients with chronic renal failure who were undergoing hemodialysis.
Studies have shown, that serum levels of Proguanylin has rise in patients with Cohn syndrome therefore it could be used as a novel marker in diagnostic and therapy of cardiac failure.