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Proguanylin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Guanylate cyclase activator 2A, Guanylate cyclase-activating protein 1, Guanylate cyclase-activating protein I, GCAP-I, GUCA2A, GUCA2
  • Species:Human
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Clearance sale RD191046100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma (EDTA, citrate, heparin)

Sample Requirements

20 µl/well

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.31–10 ng/ml

Limit of Detection

0.06 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 5.2 %

Inter-assay (Run-to-Run)

n = 6; CV = 4.1 %

Spiking Recovery

95.2 %

Dilutation Linearity

97,10%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures total proguanylin in serum, plasma
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Others, Renal disease

Summary

Proguanylin, the 116-amino acid prohormone, is a bioactive form of human guanylin that acts on intestinal guanylate cyclase, thereby regulating intestinal fluid and elektrolyte transport through the second messenger, cyclic GMP (cGMP). The cGMP increase inhibits salt absorption and stimulates chloride secretion into the gut. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to diarrhea and dehydratation characteristic of enterotoxin activity.
Proguanylin is found in circulation and plays an endocrine role by regulating the function of tissues such as the kidney and liver. Proguanylin is a significant marker in renal insufficiency. Plasma levels of proguanylin increase in patients with chronic renal failure who were undergoing hemodialysis.
Studies have shown, that serum levels of Proguanylin has rise in patients with Cohn syndrome therefore it could be used as a novel marker in diagnostic and therapy of cardiac failure.

References to Product

References

  • Kaar G, Dieplinger B, Gabriel C, Haltmayer M, Mueller T. Proguanylin and prouroguanylin--assay evaluation and clinical analyte characterization. Clin Chim Acta. 2011 Nov 20;412 (23-24):2277-83
  • Solichova P, Stejskal D, Proskova J. Guanylins - Agents with natriuretic effect. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub . Jul;150(1):85-7 (2006)
  • Stejskal D, Solichova P, Lacnak B, Vaclavik J, Hanulova Z, Humenanska V, Sprongl L, Karpisek M. Proguanylin: development, analytical characterization, and clinical testing of a new ELISA. Gen Physiol Biophys . Mar;26(1):62-5 (2007)
References to Summary

References to Proguanylin

  • Fan X, Wang Y, London R M, Eber S L, Kruse W J, Freeman R H, and Forte L R: Signaling Pathways for Guanylin and Uroguanylin in the digestive, Renal, Central Nervous, Reproductive, and Lymphoid Systems. Endocrinology 138, 4636–4648 (1997)
  • Garcia k Ch, Sauvage F J, Strubles M, Henzel W, Reilly D, and Goeddel D V: Processing and Charecterization of Human Proguanylin Expressed in Escherichia coli. The Journal of Biological Chemistry 268, 22397–22401 (1993)
  • Hamra F K, Fan X, Krause W J, et al: Prouroguanylin and Proguanylin: Purification from Colon, Structure, and Modulation of Bioactivyity by Proteases. Endocrinology 137, 257–265 (1996)
  • Rudolph J A, Hawkins J A, and Cohen M B: Proguanylin secretion and role of negative – feedback inhibition in villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol 238 G695-G702 (2002)
  • Lauber T, Neudecker P, Rosch P, and Marx U C: Solution Structure of Human Proguanylin. The Journal of Biological Chemistry 278, 24118–24124 (2003)
  • Lauber T, Nourse A, Schulz A, and Marx U C: Native and Recombinant Proguanylin Feature Identical Biophysical Properties and Are Monomeric in Solution. Biochemistry 41, 14602–14612 (2002)
  • Sauvage J F, Keshav S, Kuang W-J, Gillett N, Henzel W, and Goeddel D V: Precursor structure, expression, and tissue distribution of human guanylin. Biochemistry 89, 9089–9093 (1992)
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