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Manufactured by BioVendor

Proinsulin Intact Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Human
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Cat. No. Size Price


RZ193094100 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

100 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0–100 pmol/l

Limit of Detection

0.60 pmol/l

Intra-assay (Within-Run)

CV < 3.2%

Inter-assay (Run-to-Run)

CV < 3.2%

Spiking Recovery

102,80%

Dilutation Linearity

105,00%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
  • pig Yes
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than two hours.
  • The kit measures total intact human proinsulin in serum.
  • Calibrators are human serum based.
  • No dilution of serum samples.
  • Components of the kit are in the lyophilized, concentrated and ready-to-use states.
  • Convenient for automatization.
  • Proinsulin form, no crossreaction with insulin, C-peptide and “des” and “split” forms.

Research topic

Diabetology - Insulin, C-Peptide, Proinsulin

Summary

Proinsulin (PI), a polypeptide of 9390 MW (86 amino acids) is synthetized in the β cells of the Islets of Langerhans in the pancreas. The sequence of proinsulin is highly conserved in mammalian species and is homologous with IGF-1 and IGF-2.
This protein is processed to C-peptide and insulin forms. Both are secreted in equimolar amounts into the blood. In normal individuals, proinsulin is present in the circulatioon in very low concentrations (typical basal values 2-6 pmol/l). The level of proinsulin in serum can be a reflection of β cell status and a consequence of disfunction of PI processing and/or secretion. Proinsulin like material is increased in clinical conditions as insulinoma, familial hyperinsulinemia, non-insulin dependent diabetes mellitus.

Clinical applications:
Proinsulin is the precursor of insulin, which is the principal hormone responsible for the control of glucose metabolism. The measurement of Proinsulin in serum provides useful valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations.

Summary References (10)

References to Proinsulin

  • Cohen RM, Given BD, Licinio-Paixao J, Provow SA, Rue PA, Frank BH, Root MA, Polonsky KS, Tager HS, Rubenstein AH. Proinsulin radioimmunoassay in the evaluation of insulinomas and familial hyperproinsulinemia. Metabolism. 1986 Dec;35 (12):1137-46
  • Haffner SM, Bowsher RR, Mykkanen L, Hazuda HP, Mitchell BD, Valdez RA, Gingerich R, Monterossa A, Stern MP. Proinsulin and specific insulin concentration in high- and low-risk populations for NIDDM. Diabetes. 1994 Dec;43 (12):1490-3
  • Hanley AJ, D'Agostino R Jr, Wagenknecht LE, Saad MF, Savage PJ, Bergman R, Haffner SM. Increased proinsulin levels and decreased acute insulin response independently predict the incidence of type 2 diabetes in the insulin resistance atherosclerosis study. Diabetes. 2002 Apr;51 (4):1263-70
  • Houssa P, Dinesen B, Deberg M, Frank BH, Van Schravendijk C, Sodoyez-Goffaux F, Sodoyez JC. First direct assay for intact human proinsulin. Clin Chem. 1998 Jul;44 (7):1514-9
  • Kitabchi AE. Proinsulin and C-peptide: a review. Metabolism. 1977 May;26 (5):547-87
  • Klee GG. Human anti-mouse antibodies. Arch Pathol Lab Med. 2000 Jun;124 (6):921-3
  • Kricka LJ. Human anti-animal antibody interferences in immunological assays. Clin Chem. 1999 Jul;45 (7):942-56
  • Linde S, Roder ME, Hartling SG, Binder C, Welinder BS. Separation and quantitation of serum proinsulin and proinsulin intermediates in humans. J Chromatogr. 1991 Jul 12;548 (1-2):371-80
  • Robbins DC, Tager HS, Rubenstein AH. Biologic and clinical importance of proinsulin. N Engl J Med. 1984 May 3;310 (18):1165-75
  • Stiles AD, D'Ercole AJ. The insulin-like growth factors and the lung. Am J Respir Cell Mol Biol. 1990 Aug;3 (2):93-100
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