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Prolactin Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Lactotropin, PRL, Luteotropic hormone, Lactogenic hormone, Lactogen, HPRL
  • Species:Rat
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Cat. No. Size Price

RTC006R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody



Sample Requirements

25 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

5–80 ng/ml

Limit of Detection

0.6 ng/ml


Research topic

Reproduction, Animal studies


Rat prolactin (rPRL) is a single-chain polypeptide hormone of the rat anterior pituitary with a molecule mass of approximately 23,000. Prolactin from different species exhibits significant variations in the amino acid sequence. Rat prolactin differs from human prolactin at about 50 percent of all residues.
The secretion of rPRL from the pituitary is inhibited by hypothalamic prolactin-inhibitory factor (PIF). Although dopamine was long thought to be this PIF molecule, today it seems that there is a special peptide with prolactin-inhibiting activities.
The release of prolactin is certainly stimulated by different peptides, particularly thyrotropin releasing hormone (TRH) and vasoactive intestinal peptide (VIP). There is also evidence that rat posterior pituitary lobe contains a special prolactin releasing hormone.
The most important role of prolactin is stimulation of mammary gland growth and lactation. During pregnancy, blood prolactin levels climb, but the increases can differ enormously between rats. High prolactin levels are observed during lactation. Prolactin has a wide variety of other physiological actions, for example on the ovary. In the rat, prolactin has a luteotrophic effect which is not seen in many other species. Furthermore, prolactin is a stress hormone.
In rats, as in humans, prolactin exhibits a sleep-related diurnal variation. Peak values are seen in the late afternoon and nadir values in the morning.
Because of the variety of its actions, prolactin is one of the preferred hormones to monitor when testing the influence of new therapeutic agents and drugs on the endocrine system in the rat.

Product References (1)


  • Awodele O, Momoh AA, Awolola NA, Kale OE, Okunowo WO. The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stressinvolvement in wistar rats. Toxicol Rep. 2016 Jul 19;3:620-627. doi:10.1016/j.toxrep.2016.06.007. eCollection 2016. PubMed PMID: 28959585; PubMedCentral PMCID: PMC5615933. See more on PubMed
Summary References (25)

References to Prolactin

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  • Bonhoff A, Vuille JC, Gomez F, Gellersen B. Identification of macroprolactin in a patient with asymptomatic hyperprolactinemia as a stable PRL-IgG complex. Exp Clin Endocrinol Diabetes. 1995;103 (4):252-5
  • Cavaco B, Leite V, Santos MA, Arranhado E, Sobrinho LG. Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma. J Clin Endocrinol Metab. 1995 Aug;80 (8):2342-6
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  • Linzer DI, Nathans D. Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family. Proc Natl Acad Sci U S A. 1984 Jul;81 (14):4255-9
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  • Shiu RP, Iwasiow BM. Prolactin-inducible proteins
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