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Manufactured by BioVendor

Prolactin Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Rat
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Cat. No. Size Price


RTC006R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

5–80 ng/ml

Limit of Detection

0.6 ng/ml

Summary

Research topic

Animal studies

Summary

Rat prolactin (rPRL) is a single-chain polypeptide hormone of the rat anterior pituitary with a molecule mass of approximately 23,000. Prolactin from different species exhibits significant variations in the amino acid sequence. Rat prolactin differs from human prolactin at about 50 percent of all residues.
The secretion of rPRL from the pituitary is inhibited by hypothalamic prolactin-inhibitory factor (PIF). Although dopamine was long thought to be this PIF molecule, today it seems that there is a special peptide with prolactin-inhibiting activities.
The release of prolactin is certainly stimulated by different peptides, particularly thyrotropin releasing hormone (TRH) and vasoactive intestinal peptide (VIP). There is also evidence that rat posterior pituitary lobe contains a special prolactin releasing hormone.
The most important role of prolactin is stimulation of mammary gland growth and lactation. During pregnancy, blood prolactin levels climb, but the increases can differ enormously between rats. High prolactin levels are observed during lactation. Prolactin has a wide variety of other physiological actions, for example on the ovary. In the rat, prolactin has a luteotrophic effect which is not seen in many other species. Furthermore, prolactin is a stress hormone.
In rats, as in humans, prolactin exhibits a sleep-related diurnal variation. Peak values are seen in the late afternoon and nadir values in the morning.
Because of the variety of its actions, prolactin is one of the preferred hormones to monitor when testing the influence of new therapeutic agents and drugs on the endocrine system in the rat.

Product References (1)

References

  • Awodele O, Momoh AA, Awolola NA, Kale OE, Okunowo WO. The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stressinvolvement in wistar rats. Toxicol Rep. 2016 Jul 19;3:620-627. doi:10.1016/j.toxrep.2016.06.007. eCollection 2016. PubMed PMID: 28959585; PubMedCentral PMCID: PMC5615933. See more on PubMed
Summary References (25)

References to Prolactin

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  • Aubert ML, Grumbach MM, Kaplan SL. Heterologous radioimmunoassay for plasma human prolactin (hPRL); values in normal subjects, puberty, pregnancy and in pituitary disorders. Acta Endocrinol (Copenh). 1974 Nov;77 (3):460-76
  • Bonhoff A, Vuille JC, Gomez F, Gellersen B. Identification of macroprolactin in a patient with asymptomatic hyperprolactinemia as a stable PRL-IgG complex. Exp Clin Endocrinol Diabetes. 1995;103 (4):252-5
  • Cavaco B, Leite V, Santos MA, Arranhado E, Sobrinho LG. Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma. J Clin Endocrinol Metab. 1995 Aug;80 (8):2342-6
  • Cowden EA, Ratcliffe WA, Beastall GH, Ratcliffe JG. Laboratory assessment of prolactin status. Ann Clin Biochem. 1979 May;16 (3):113-21
  • Fahie-Wilson MN. Detection of macroprolactin causing hyperprolactinemia in commercial assays for prolactin. Clin Chem. 2000 Dec;46 (12):2022-3
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  • Frantz AG. Prolactin. N Engl J Med. 1978 Jan 26;298 (4):201-7
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  • Friesen H, Guyda H, Hwang P. 2,3-diphosphoglycerate affects enzymes of glucose metabolism in red blood cells. Nat New Biol. 1971 Jul 7;232 (27):19-20
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  • Hwang P, Guyda H, Friesen H. Purification of human prolactin. J Biol Chem. 1972 Apr 10;247 (7):1955-8
  • Jacobs LS, Snyder PJ, Wilber JF, Utiger RD, Daughaday WH. Increased serum prolactin after administration of synthetic thyrotropin releasing hormone (TRH) in man. J Clin Endocrinol Metab. 1971 Dec;33 (6):996-8
  • Kelly PA, Djiane J, Katoh M, Ferland LH, Houdebine LM, Teyssot B, Dusanter-Fourt I. The interaction of prolactin with its receptors in target tissues and its mechanism of action. Recent Prog Horm Res. 1984;40:379-439
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  • Kleinberg DL, Frantz AG. Human prolactin: measurement in plasma by in vitro bioassay. J Clin Invest. 1971 Aug;50 (8):1557-68
  • Lewis UJ, Singh RN, Seavey BK. Human prolactin: isolation and some properties. Biochem Biophys Res Commun. 1971 Sep;44 (5):1169-76
  • Linzer DI, Nathans D. Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family. Proc Natl Acad Sci U S A. 1984 Jul;81 (14):4255-9
  • McLeod. RM. Regulation of Prolactin Secretion: In Frontiers in Neuroendocrinology (Martin L. and Garrong N.F. eds). Raven Press. 164,1976;
  • Nikolics K, Mason AJ, Szonyi E, Ramachandran J, Seeburg PH. A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone. Nature. 1985 Aug 8-14;316 (6028):511-7
  • Rasmussen C, Bergh T, Wide L, Brownell J. CV 205-502: a new long-acting drug for inhibition of prolactin hypersecretion. Clin Endocrinol (Oxf). 1987 Mar;26 (3):321-6
  • Reyes FI, Winter JS, Faiman C. Pituitary-ovarian relationships preceding the menopause. I. A cross-sectional study of serum follice-stimulating hormone, luteinizing hormone, prolactin, estradiol, and progesterone levels. Am J Obstet Gynecol. 1977 Nov 1;129 (5):557-64
  • Schneider W, Marcovitz S, Al-Shammari S, Yago S, Chevalier S. Reactivity of macroprolactin in common automated immunoassays. Clin Biochem. 2001 Sep;34 (6):469-73
  • Shiu RP, Iwasiow BM. Prolactin-inducible proteins
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