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Q-Plex™ Human Cytokine-Inflammation (9-plex)

  • Regulatory status:RUO
  • Type:Multiplex Assays
  • Other names:Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), Interleukin-1α
  • Species:Human
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Cat. No. Size Price

110433HU 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RQS110449HU to 110433HU


Multiplex Assays


Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well for all nine analytes in the panel


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.

Calibration Range

  • IL-1α : 4000 – 5.5 pg/ml
  • IL-1β : 10000 – 13.7 pg/ml
  • IL-2 : 4000 – 5.5 pg/ml
  • IL-4 : 2000 – 2.7 pg/ml - IL-6 : 3000 – 4.1 pg/ml
  • IL-8 : 2000 – 2.7 pg/ml - IL-10 : 3000 – 4.1 pg/ml
  • IFNγ : 8 00 – 11.0 pg/ml
  • TNFα : 4000 – 5.5 pg/ml Actual values may vary from kit to kit. Please see the antigen card included in your kit for specific values.

Limit of Detection

  • IL-1α : 0.27 pg/ml
  • IL-1β : 2.0 pg/ml
  • IL-2 : 0.72 pg/ml
  • IL-4 : 0.24 pg/ml
  • IL-6 : 0.55 pg/ml
  • IL-8 : 0.42 pg/ml
  • IL-10 : 0.30 pg/ml
  • IFNγ : 1.18 pg/ml
  • TNFα : 1.36 pg/ml


  • For research use only
  • Detection Method: Chemiluminescent
  • Analytes: IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IFNγ, TNFα
  • This kit is validated for use with plasma, serum, and cell culture supernates

Research topic

Cytokines and chemokines and related molecules, Diabetology - Other Relevant Products, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Transplantation, Cardiovascular disease, Coronary artery disease, Energy metabolism and body weight regulation, Sepsis


The multiplexed ELISA kits, called Q-Plex™ measure the concentration of multiple proteins in each sample. Quantify cytokines, chemokines, hormones, and other common disease indicating proteins with standard or custom kits. Q-Plex Arrays are built by printing nano spots in defined arrays of multiple distinct capture antibodies in each well of a multiwell plate. Multiplexed ELISAs maximize data generation from each sample, and provide a low cost per data point.

Traditional Enzyme-Linked Immunosorbent assays (ELISA), have been used for decades in research and development, disease diagnosis and other critical fields in science. Multiplexed ELISAs offer the same time proven reliability with updated efficiency. Mutliplexed ELISAs have been developed to provide the sensitivity of ELISA with the ability to test for multiple analytes at the same time. With Q-Plex scientists can measure up to 25 distinct analytes in each well of a 96-well plate.

The Q-Plex™ Human Cytokine – Inflammation (9-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 2.5 hours. The Q-Plex™ Human Cytokine – Inflammation (9-plex) provides researchers an easy to use and cost effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high quality reagents help ensure the accuracy of your results.

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