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Q-Plex™ Human FSH

  • Regulatory status:RUO
  • Type:Singleplex Assays
  • Other names:Follicle Stimulating Hormone
  • Species:Human
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Cat. No. Size Price


463749HU 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RQS463749HU to 463749HU

Type

Singleplex Assays

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.

Calibration Range

167–0.69 mIU/mL

Limit of Detection

0.5 mIU/mL

Summary

Features

  • For research use only
  • Detection Method: Chemiluminescent
  • This kit is validated for use with plasma, serum, and cell culture supernates

Research topic

Reproduction

Summary

The Q-Plex™ Human FSH Kit is a fully quantitative ELISA. The assay measurement is achieved by placing two spots consisting of capture antibodies in a defined array to the bottom of each well of a 96-well plate. Along with the assay spots, there is one positive control spot for assuring proper assay procedure and for software overlay placement. High quality reagents help ensure the accuracy and precision of your results.

Using less than 50 μL of sample per well, up to 80 samples can be assayed for a single assay within 2.5 hours. The array design of the singleplex kit, allows the user to have intra-well replicates for additional statistical data. The Q-Plex™ Human FSH ELISA provides researchers an easy to use and cost effective means of generating data for each sample.

Summary References (15)

References to FSH

  • Butt WR, Ryle M, Shirley A. Kinetic studies with 125I-labelled follicle-stimulating hormone. J Endocrinol. 1973 Aug;58 (2):275-87
  • Cohen KL. Metabolic, endocrine, and drug-induced interference with pituitary function tests: a review. Metabolism. 1977 Oct;26 (10):1165-77
  • Jeffcoate SL. The control of testicular function in the adult. Clin Endocrinol Metab. 1975 Nov;4 (3):521-43
  • Jockenhovel F, Khan SA, Nieschlag E. Circulating antibodies to monoclonal immunoglobulins used in a follitropin assay may cause incorrect fertility diagnosis. J Clin Chem Clin Biochem. 1989 Oct;27 (10):825-8
  • Kjeld JM, Harsoulis P, Kuku SF, Marshall JC, Kaufman B, Fraser TR. Infusions of hFSH and hLH in normal men. I. Kinetics of human follicle stimulating hormone. Acta Endocrinol (Copenh). 1976 Feb;81 (2):225-33
  • Knobil E. The neuroendocrine control of the menstrual cycle. Recent Prog Horm Res. 1980;36:53-88
  • Leonard JM, Leach RB, Couture M, Paulsen CA. Plasma and urinary follicle-stimulating hormone levels in oligospermia. J Clin Endocrinol Metab. 1972 Jan;34 (1):209-14
  • Lundy LE, Lee SG, Levy W, Woodruff JD, Wu CH, Abdalla M. The ovulatory cycle. A histologic, thermal, steroid, and gonadotropin correlation. Obstet Gynecol. 1974 Jul;44 (1):14-25
  • Marshall JC. Clinics in endocrinology and metabolism. Investigative procedures. Clin Endocrinol Metab. 1975 Nov;4 (3):545-67
  • Rebar RW, Erickson GF, Yen SS. Idiopathic premature ovarian failure: clinical and endocrine characteristics. Fertil Steril. 1982 Jan;37 (1):35-41
  • Reiter EO, Kulin HE. Suppressed follicle stimulating hormone in men with chorionic gonadotropin secreting testicular tumors. J Clin Endocrinol Metab. 1971 Dec;33 (6):957-61
  • Seth J, Hanning I, Bacon RR, Hunter WM. Progress and problems in immunoassays for serum pituitary gonadotrophins: evidence from the UK external quality assessment schemes, (EQAS) 1980-1988. Clin Chim Acta. 1989 Dec 29;186 (1):67-82
  • Shome B, Parlow AF. Human follicle stimulating hormone (hFSH): first proposal for the amino acid sequence of the alpha-subunit (hFSHa) and first demonstration of its identity with the alpha-subunit of human luteinizing hormone (hLHa). J Clin Endocrinol Metab. 1974 Jul;39 (1):199-202
  • Shome B, Parlow AF. Human follicle stimulating hormone: first proposal for the amino acid sequence of the hormone-specific, beta subunit (hFSHb). J Clin Endocrinol Metab. 1974 Jul;39 (1):203-5
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
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