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Q-Plex™ Human Resistin

  • Regulatory status:RUO
  • Type:Singleplex Assays
  • Other names:Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RETN, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199
  • Species:Human
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Cat. No. Size Price


469149HU 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RQS469149HU to 469149HU

Type

Singleplex Assays

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.

Calibration Range

10000–89.16 pg/mL

Limit of Detection

3.2 pg/mL

Summary

Features

  • For research use only
  • Detection Method: Chemiluminescent
  • The kit measures Human Resistin
  • This kit is validated for use with plasma, serum, and cell culture supernates
  • Wide range across the standard curve means fewer dilutions per sample.
  • Better sensitivity than traditional ELISAs capture low analyte levels.
  • High end range captures data for concentrated samples.
  • Intra-well precision from replicate micro-spots provides greater assurity of results
  • Easy to use ELISA protocol

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary

The Q-Plex™ Human Resistin Kit is a fully quantitative ELISA. The assay measurement is achieved by placing two spots consisting of capture antibodies in a defined array to the bottom of each well of a 96-well plate. Along with the assay spots, there is one positive control spot for assuring proper assay procedure and for software overlay placement. High quality reagents help ensure the accuracy and precision of your results.

Using less than 50 μL of sample per well, up to 80 samples can be assayed for a single assay within 2.5 hours. The array design of the singleplex kit, allows the user to have intra-well replicates for additional statistical data. The Q-Plex™ Human Resistin ELISA provides researchers an easy to use and cost effective means of generating data for each sample.

Summary References (21)

References to Resistin

  • Banerjee RR, Lazar MA. Dimerization of resistin and resistin-like molecules is determined by a single cysteine. J Biol Chem. 2001 Jul 13;276 (28):25970-3
  • Bokarewa M, Nagaev I, Dahlberg L, Smith U, Tarkowski A. Resistin, an adipokine with potent proinflammatory properties. J Immunol. 2005 May 1;174 (9):5789-95
  • Fasshauer M, Klein J, Neumann S, Eszlinger M, Paschke R. Tumor necrosis factor alpha is a negative regulator of resistin gene expression and secretion in 3T3-L1 adipocytes. Biochem Biophys Res Commun. 2001 Nov 9;288 (4):1027-31
  • Fasshauer M, Paschke R. Regulation of adipocytokines and insulin resistance. Diabetologia. 2003 Dec;46 (12):1594-603
  • Gong H, Ni Y, Guo X, Fei L, Pan X, Guo M, Chen R. Resistin promotes 3T3-L1 preadipocyte differentiation. Eur J Endocrinol. 2004 Jun;150 (6):885-92
  • Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA:. Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002) .
  • Juan CC, Au LC, Fang VS, Kang SF, Ko YH, Kuo SF, Hsu YP, Kwok CF, Ho LT. Suppressed gene expression of adipocyte resistin in an insulin-resistant rat model probably by elevated free fatty acids. Biochem Biophys Res Commun. 2001 Dec 21;289 (5):1328-33
  • Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. J Clin Endocrinol Metab. 2004 Jun;89 (6):2548-56
  • Kim KH, Lee K, Moon YS, Sul HS. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation. J Biol Chem. 2001 Apr 6;276 (14):11252-6
  • Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T:. Low resistin levels in adipose tissues and serum high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Archiv Biochem Biophys. 416, 164-170 (2003) .
  • Malo E, Ukkola O, Jokela M, Moilanen L, Kahonen M, Nieminen MS, Salomaa V, Jula A, Kesaniemi YA. Resistin is an indicator of the metabolic syndrome according to five different definitions in the Finnish Health 2000 survey. Metab Syndr Relat Disord. 2011 Jun;9 (3):203-10
  • Meier U, Gressner AM. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem. 2004 Sep;50 (9):1511-25
  • Milan G, Granzotto M, Scarda A, Calcagno A, Pagano C, Federspil G, Vettor R. Resistin and adiponectin expression in visceral fat of obese rats: effect of weight loss. Obes Res. 2002 Nov;10 (11):1095-103
  • Nogueiras R, Gallego R, Gualillo O, Caminos JE, Garcia-Caballero T, Casanueva FF, Dieguez C. Resistin is expressed in different rat tissues and is regulated in a tissue- and gender-specific manner. FEBS Lett. 2003 Jul 31;548 (1-3):21-7
  • Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW:. Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001) .
  • Rajala MW, Obici S, Scherer PE, Rossetti L. Adipose-derived resistin and gut-derived resistin-like molecule-beta selectively impair insulin action on glucose production. J Clin Invest. 2003 Jan;111 (2):225-30
  • Sadashiv, Tiwari S, Paul BN, Kumar S, Chandra A, Dhananjai S, Negi MP. Over expression of resistin in adipose tissue of the obese induces insulin resistance. World J Diabetes. 2012 Jul 15;3 (7):135-41
  • Satoh H, Nguyen MT, Miles PD, Imamura T, Usui I, Olefsky JM. Adenovirus-mediated chronic "hyper-resistinemia" leads to in vivo insulin resistance in normal rats. J Clin Invest. 2004 Jul;114 (2):224-31
  • Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA:. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
  • Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA:. A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001) .
  • Steppan CM, Lazar MA. The current biology of resistin. J Intern Med. 2004 Apr;255 (4):
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