Select country change
Shopping cart (0 , 0,00 ) Menu Search
Distributed product

Q-Plex™ Human TNFβ HS

  • Regulatory status:RUO
  • Type:Singleplex Assays
  • Other names:Lymphotoxin-alpha, TNF superfamily member 1
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


461649HU 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RQS461649HU to 461649HU

Type

Singleplex Assays

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.

Calibration Range

470–0.64 pg/mL

Limit of Detection

0.57 pg/mL

Summary

Features

  • For research use only
  • Detection Method: Chemiluminescent
  • The kit measures Human TNFβ HS
  • This kit is validated for use with plasma, serum, and cell culture supernates
  • Wide range across the standard curve means fewer dilutions per sample.
  • Better sensitivity than traditional ELISAs capture low analyte levels.
  • High end range captures data for concentrated samples.
  • Intra-well precision from replicate micro-spots provides greater assurity of results
  • Easy to use ELISA protocol

Research topic

Cytokines and chemokines and related molecules

Summary

The Q-Plex™ Human TNFβ HS Kit is a fully quantitative ELISA. The assay measurement is achieved by placing two spots consisting of capture antibodies in a defined array to the bottom of each well of a 96-well plate. Along with the assay spots, there is one positive control spot for assuring proper assay procedure and for software overlay placement. High quality reagents help ensure the accuracy and precision of your results.

Using less than 50 μL of sample per well, up to 80 samples can be assayed for a single assay within 2.5 hours. The array design of the singleplex kit, allows the user to have intra-well replicates for additional statistical data. The Q-Plex™ Human TNFβ HS ELISA provides researchers an easy to use and cost effective means of generating data for each sample.

Summary References (20)

References to TNF-beta

  • Adolf GR, Apfler I. A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
  • Bertolini DR, Nedwin GE, Bringman TS, Smith DD, Mundy GR. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6-12;319 (6053):516-8
  • Beutler B, Cerami A. Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
  • Broudy VC, Harlan JM, Adamson JW. Disparate effects of tumor necrosis factor-alpha/cachectin and tumor necrosis factor-beta/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J Immunol. 1987 Jun 15;138 (12):4298-302
  • Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
  • Clark IA, Gray KM, Rockett EJ, Cowden WB, Rockett KA, Ferrante A, Aggarwal BB. Increased lymphotoxin in human malarial serum, and the ability of this cytokine to increase plasma interleukin-6 and cause hypoglycaemia in mice: implications for malarial pathology. Trans R Soc Trop Med Hyg. 1992 Nov-Dec;86 (6):602-7
  • Exley AR, Cohen J. Optimal collection of blood samples for the measurement of tumor necrosis factor alpha. Cytokine. 1990 Sep;2 (5):353-6
  • Gray PW, Aggarwal BB, Benton CV, Bringman TS, Henzel WJ, Jarrett JA, Leung DW, Moffat B, Ng P, Svedersky LP, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature. 1984 Dec 20-1985 Jan 2;312 (5996):721-4
  • Himmler A, Maurer-Fogy I, Kronke M, Scheurich P, Pfizenmaier K, Lantz M, Olsson I, Hauptmann R, Stratowa C, Adolf GR. Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
  • Ishibashi K, Ishitsuka K, Chuman Y, Otsuka M, Kuwazuru Y, Iwahashi M, Utsunomiya A, Hanada S, Sakurami T, Arima T. Tumor necrosis factor-beta in the serum of adult T-cell leukemia with hypercalcemia. Blood. 1991 Jun 1;77 (11):2451-5
  • Kapas L, Krueger JM. Tumor necrosis factor-beta induces sleep, fever, and anorexia. Am J Physiol. 1992 Sep;263 (3 Pt 2):R703-7
  • Kramer SM, Carver ME. Serum-free in vitro bioassay for the detection of tumor necrosis factor. J Immunol Methods. 1986 Nov 6;93 (2):201-6
  • Lamche HR, Adolf GR. Highly sensitive enzyme immunoassays for antibodies to human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta). J Immunol Methods. 1990 Aug 7;131 (2):283-9
  • Locksley RM, Heinzel FP, Shepard HM, Agosti J, Eessalu TE, Aggarwal BB, Harlan JM. Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
  • Old LJ. Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
  • Paul NL, Ruddle NH. Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
  • Picarella DE, Kratz A, Li CB, Ruddle NH, Flavell RA. Insulitis in transgenic mice expressing tumor necrosis factor beta (lymphotoxin) in the pancreas. Proc Natl Acad Sci U S A. 1992 Nov 1;89 (21):10036-40
  • Scheurich P, Thoma B, Ucer U, Pfizenmaier K. Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
  • Shalaby MR, Aggarwal BB, Rinderknecht E, Svedersky LP, Finkle BS, Palladino MA Jr. Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J Immunol. 1985 Sep;135 (3):2069-73
  • Spies T, Morton CC, Nedospasov SA, Fiers W, Pious D, Strominger JL. Genes for the tumor necrosis f
Related Products Docs