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Q-Plex™ Human TNFRII

  • Regulatory status:RUO
  • Type:Singleplex Assays
  • Other names:Tumor necrosis factor receptor 2, TNFR2, CD120b
  • Species:Human
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Cat. No. Size Price


460049HU 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RQS460049HU to 460049HU

Type

Singleplex Assays

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.

Calibration Range

2000–2.74 pg/mL

Limit of Detection

1.5 pg/mL

Summary

Features

  • For research use only
  • Detection Method: Chemiluminescent
  • The kit measures Human TNFRII
  • This kit is validated for use with plasma, serum, and cell culture supernates
  • Wide range across the standard curve means fewer dilutions per sample.
  • Better sensitivity than traditional ELISAs capture low analyte levels.
  • High end range captures data for concentrated samples.
  • Intra-well precision from replicate micro-spots provides greater assurity of results
  • Easy to use ELISA protocol

Research topic

Cytokines and chemokines and related molecules

Summary

The Q-Plex™ Human TNFRII Kit is a fully quantitative ELISA. The assay measurement is achieved by placing two spots consisting of capture antibodies in a defined array to the bottom of each well of a 96-well plate. Along with the assay spots, there is one positive control spot for assuring proper assay procedure and for software overlay placement. High quality reagents help ensure the accuracy and precision of your results.

Using less than 50 μL of sample per well, up to 80 samples can be assayed for a single assay within 2.5 hours. The array design of the singleplex kit, allows the user to have intra-well replicates for additional statistical data. The Q-Plex™ Human TNFRII ELISA provides researchers an easy to use and cost effective means of generating data for each sample.

Summary References (17)

References to TNF-R (80 kDa)

  • Aderka D, Wysenbeek A, Engelmann H, Cope AP, Brennan F, Molad Y, Hornik V, Levo Y, Maini RN, Feldmann M, et al. Correlation between serum levels of soluble tumor necrosis factor receptor and disease activity in systemic lupus erythematosus. Arthritis Rheum. 1993 Aug;36 (8):1111-20
  • Adolf GR, Apfler I. A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
  • Aggarwal BB, Eessalu TE, Hass PE. Characterization of receptors for human tumour necrosis factor and their regulation by gamma-interferon. Nature. 1985 Dec 19-1986 Jan 1;318 (6047):665-7
  • Andus T, Gross V, Holstege A, Ott M, Weber M, David M, Gallati H, Gerok W, Scholmerich J. High concentrations of soluble tumor necrosis factor receptors in ascites. Hepatology. 1992 Sep;16 (3):749-55
  • Austgulen R, Liabakk NB, Lien E, Espevik T. Increased levels of soluble tumor necrosis factor-alpha receptors in serum from pregnant women and in serum and urine samples from newborns. Pediatr Res. 1993 Jan;33 (1):82-7
  • Baglioni C, McCandless S, Tavernier J, Fiers W. Binding of human tumor necrosis factor to high affinity receptors on HeLa and lymphoblastoid cells sensitive to growth inhibition. J Biol Chem. 1985 Nov 5;260 (25):13395-7
  • Barrera P, Boerbooms AM, Janssen EM, Sauerwein RW, Gallati H, Mulder J, de Boo T, Demacker PN, van de Putte LB, van der Meer JW. Circulating soluble tumor necrosis factor receptors, interleukin-2 receptors, tumor necrosis factor alpha, and interleukin-6 levels in rheumatoid arthritis. Longitudinal evaluation during methotrexate and azathioprine therapy. Arthritis Rheum. 1993 Aug;36 (8):1070-9
  • Baumann P, Romero R, Berry S, Gomez R, McFarlin B, Araneda H, Cotton DB, Fidel P. Evidence of participation of the soluble tumor necrosis factor receptor I in the host response to intrauterine infection in preterm labor. Am J Reprod Immunol. 1993 Sep-Oct;30 (2-3):184-93
  • Beutler B, Cerami A. Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
  • Beutler B, Mahoney J, Le Trang N, Pekala P, Cerami A. Purification of cachectin, a lipoprotein lipase-suppressing hormone secreted by endotoxin-induced RAW 264.7 cells. J Exp Med. 1985 May 1;161 (5):984-95
  • Brockhaus M, Schoenfeld HJ, Schlaeger EJ, Hunziker W, Lesslauer W, Loetscher H. Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies. Proc Natl Acad Sci U S A. 1990 Apr;87 (8):3127-31
  • Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
  • Creasey AA, Yamamoto R, Vitt CR. A high molecular weight component of the human tumor necrosis factor receptor is associated with cytotoxicity. Proc Natl Acad Sci U S A. 1987 May;84 (10):3293-7
  • Godfried MH, van der Poll T, Jansen J, Romijin JA, Schattenkerk JK, Endert E, van Deventer SJ, Sauerwein HP. Soluble receptors for tumour necrosis factor: a putative marker of disease progression in HIV infection. AIDS. 1993 Jan;7 (1):33-6
  • Grosen EA, Yamamoto RS, Ioli G, Ininns EK, Gatanaga M, Gatanaga T, DiSaia PJ, Berman M, Manetta A, Granger GA. Blocking factors (soluble membrane receptors) for tumor necrosis factor and lymphotoxin detected in ascites and released in short-term cultures obtained from ascites and solid tumors in women with gynecologic malignancy. Lymphokine Cytokine Res. 1992 Dec;11 (6):347-53
  • Gruss HJ, Dolken G, Brach MA, Mertelsmann R, Herrmann F. The significance of serum levels of soluble 60kDa receptors for tumor necrosis factor in patients with Hodgkin's disease. Leukemia. 1993 Sep;7 (9):1339-43
  • Heilig B, Fiehn C, Brockhaus M, Gallati H, Pezzutto A, Hunstein W. Evaluation of soluble tumor necrosis factor (TNF) receptors and TNF receptor antibodies in patients
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