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Q-Plex™ Mouse Cytokine-Screen (16-plex)

  • Regulatory status:RUO
  • Type:Multiplex Assays
  • Other names:Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), Interleukin-1α
  • Species:Mouse
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Cat. No. Size Price


110949MS 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RQS110949MS to 110949MS

Type

Multiplex Assays

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well for all sixteen analytes in the panel

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.

Limit of Detection

  • IL-1α : 0.89 pg/ml
  • IL-1β : 1.48 pg/ml
  • IL-2 : 0.34 pg/ml
  • IL-3 : 0.83 pg/ml
  • IL-4 : 0.27 pg/ml
  • IL-5 : 0.48 pg/ml
  • IL-6 : 0.07 pg/ml
  • IL-10 : 0.84 pg/ml
  • IL-12p70 : 0.47 pg/ml
  • IL-17 : 0.70 pg/ml
  • IMCP-1 : 1.21 pg/ml
  • IFNγ : 0.19 pg/ml
  • TNFα : 1.12 pg/ml
  • MIP-1α : 0.15 pg/ml
  • GMCSF : 0.33 pg/ml
  • RANTES : 0.26 pg/ml
Summary

Features

  • For research use only
  • Detection Method: Chemiluminescent
  • Analytes: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, IFNγ, TNFα, MIP-1α, GMCSF, RANTES
  • This kit is validated for use with plasma, serum, and cell culture supernates

Research topic

Cytokines and chemokines and related molecules, Diabetology - Other Relevant Products, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Transplantation, Animal studies, Cardiovascular disease, Coronary artery disease, Energy metabolism and body weight regulation, Sepsis, Chemokines, Immunology

Summary

The multiplexed ELISA kits, called Q-Plex™ measure the concentration of multiple proteins in each sample. Quantify cytokines, chemokines, hormones, and other common disease indicating proteins with standard or custom kits. Q-Plex Arrays are built by printing nano spots in defined arrays of multiple distinct capture antibodies in each well of a multiwell plate. Multiplexed ELISAs maximize data generation from each sample, and provide a low cost per data point.

Traditional Enzyme-Linked Immunosorbent assays (ELISA), have been used for decades in research and development, disease diagnosis and other critical fields in science. Multiplexed ELISAs offer the same time proven reliability with updated efficiency. Mutliplexed ELISAs have been developed to provide the sensitivity of ELISA with the ability to test for multiple analytes at the same time. With Q-Plex scientists can measure up to 25 distinct analytes in each well of a 96-well plate.

The Q-Plex™ Mouse Cytokine – Screen (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.5 hours. The Mouse Cytokine – Screen (16-Plex) provides researchers an easy to use and cost effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high quality reagents help ensure the accuracy of your results.

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