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Q-PlexTM Human Micronutrient Array (7-plex)

  • Regulatory status:RUO
  • Type:Multiplex Assays
  • Other names:AGP1, Orosomucoid-1, OMD1, ORM1
  • Species:Human
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Cat. No. Size Price


New RQS565149HU 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Multiplex Assays

Applications

Plasma

Sample Requirements

5 µl of plasma (with 45 µl of diluent) for all seven analytes in the panel

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.

Calibration Range

  • AGP : 0.36 - 0.0005 g/l
  • CRP : 5.61 - 0.0077 mg/l
  • Ferritin : 115 - 0.1578 μg/l
  • sTfR : 107 - 0.1468 mg/l
  • RBP4 : 1.01 - 0.0014 μmol/l
  • Tg : 13.7 - 0.0188 μg/l
  • HRP-2 : 0.96 - 0.0013 μg/l Actual values may vary from kit to kit. Please see the antigen card included in your kit for specific values.

Limit of Detection

  • AGP : 0.0019 g/l
  • CRP : 0.006 mg/l
  • Ferritin : 0.2057 μg/l
  • sTfR : 0.1917 mg/l
  • RBP4 : 0.0054 μmol/l
  • Tg : 0.0244 μg/l
  • HRP-2 : 0.0017 μg/l
Summary

Features

  • For research use only
  • Detection Method: Chemiluminescent
  • Analytes: AGP, CRP, Ferritin, sTfR, RBP4, Tg, HRP2
  • This kit is validated for use with plasma.

Research topic

Immune Response, Infection and Inflammation, Oncology, Sepsis, Iron metabolism, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary

The multiplexed ELISA kits, called Q-Plex™ measure the concentration of multiple proteins in each sample. Quantify cytokines, chemokines, hormones, and other common disease indicating proteins with standard or custom kits. Q-Plex Arrays are built by printing nano spots in defined arrays of multiple distinct capture antibodies in each well of a multiwell plate. Multiplexed ELISAs maximize data generation from each sample, and provide a low cost per data point.

Traditional Enzyme-Linked Immunosorbent assays (ELISA), have been used for decades in research and development, disease diagnosis and other critical fields in science. Multiplexed ELISAs offer the same time proven reliability with updated efficiency. Mutliplexed ELISAs have been developed to provide the sensitivity of ELISA with the ability to test for multiple analytes at the same time. With Q-Plex scientists can measure up to 25 distinct analytes in each well of a 96-well plate.

The Q-Plex™ Human Micronutrient Array (7-plex) is a fully quantitative chemiluminescent assay allowing concurrent measurement of biomarkers used in nutritional assessment in heparinized plasma samples. Analytes CRP, Ferritin, Thyroglobulin (Tg), and HRP2 are sandwich ELISAs while AGP, sTfR and RBP4 are competitive ELISAs. This product is alternatively known in previous publications as Multiplex Micronutrient Assessment Tool (MMAT). A Human Micronutrient Assay kit contains a 96-well plate, with each well featuring the 7-plex along with a positive control and all the other reagents necessary to perform the test. Our high quality reagents help ensure the accuracy of your results. Using just 5 µl of plasma (with 45 µl of diluent) per well, up to 80 wells can be tested for all seven markers in the panel in 2.5 hours. Q-Plex Arrays provide scientists with an easy-to-use and cost effective means of generating a biomarker profile for each sample.

References to Summary

References to Alpha-1-Acidic Glycoprotein

  • Christiansen MS, Hesse D, Ekbom P, Hesse U, Damm P, Hommel E, Feldt-Rasmussen B, Mathiesen E. Increased urinary orosomucoid excretion predicts preeclampsia in pregnant women with pregestational type 1 diabetes. Diabetes Res Clin Pract. 2010 Jul;89 (1):16-21
  • Christiansen MS, Hommel E, Magid E, Feldt-Rasmussen B. Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes. Diabetologia. 2002 Jan;45 (1):115-20
  • Harley J, Roberts R, Joyce P, Mulder R, Luty S, Frampton C, Kennedy M. Orosomucoid influences the response to antidepressants in major depressive disorder. J Psychopharmacol. 2010 Apr;24 (4):531-5
  • Ligresti G, Aplin AC, Dunn BE, Morishita A, Nicosia RF. The acute phase reactant orosomucoid-1 is a bimodal regulator of angiogenesis with time- and context-dependent inhibitory and stimulatory properties. PLoS One. 2012;7 (8):e41387
  • Sochacka J, Pawelczak B. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. Acta Pol Pharm. 2012 Jan-Feb;69 (1):161-6
  • Christiansen MS, Hesse D, Ekbom P, Hesse U, Damm P, Hommel E, Feldt-Rasmussen B, Mathiesen E. Increased urinary orosomucoid excretion predicts preeclampsia in pregnant women with pregestational type 1 diabetes. Diabetes Res Clin Pract. 2010 Jul;89 (1):16-21
  • Christiansen MS, Hommel E, Magid E, Feldt-Rasmussen B. Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes. Diabetologia. 2002 Jan;45 (1):115-20
  • Harley J, Roberts R, Joyce P, Mulder R, Luty S, Frampton C, Kennedy M. Orosomucoid influences the response to antidepressants in major depressive disorder. J Psychopharmacol. 2010 Apr;24 (4):531-5
  • Ligresti G, Aplin AC, Dunn BE, Morishita A, Nicosia RF. The acute phase reactant orosomucoid-1 is a bimodal regulator of angiogenesis with time- and context-dependent inhibitory and stimulatory properties. PLoS One. 2012;7 (8):e41387
  • Sochacka J, Pawelczak B. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. Acta Pol Pharm. 2012 Jan-Feb;69 (1):161-6
  • Christiansen MS, Hesse D, Ekbom P, Hesse U, Damm P, Hommel E, Feldt-Rasmussen B, Mathiesen E. Increased urinary orosomucoid excretion predicts preeclampsia in pregnant women with pregestational type 1 diabetes. Diabetes Res Clin Pract. 2010 Jul;89 (1):16-21
  • Christiansen MS, Hommel E, Magid E, Feldt-Rasmussen B. Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes. Diabetologia. 2002 Jan;45 (1):115-20
  • Harley J, Roberts R, Joyce P, Mulder R, Luty S, Frampton C, Kennedy M. Orosomucoid influences the response to antidepressants in major depressive disorder. J Psychopharmacol. 2010 Apr;24 (4):531-5
  • Ligresti G, Aplin AC, Dunn BE, Morishita A, Nicosia RF. The acute phase reactant orosomucoid-1 is a bimodal regulator of angiogenesis with time- and context-dependent inhibitory and stimulatory properties. PLoS One. 2012;7 (8):e41387
  • Sochacka J, Pawelczak B. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. Acta Pol Pharm. 2012 Jan-Feb;69 (1):161-6
  • Christiansen MS, Hesse D, Ekbom P, Hesse U, Damm P, Hommel E, Feldt-Rasmussen B, Mathiesen E. Increased urinary orosomucoid excretion predicts preeclampsia in pregnant women with pregestational type 1 diabetes. Diabetes Res Clin Pract. 2010 Jul;89 (1):16-21
  • Christiansen MS, Hommel E, Magid E, Feldt-Rasmussen B. Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes. Diabetologia. 2002 Jan;45 (1):115-20
  • Harley J, Roberts R, Joyce P, Mulder R, Luty S, Frampton C, Kennedy M. Orosomucoid influences the response to antidepressants in major depressive disorder. J Psychopharmacol. 2010 Apr;24 (4):531-5
  • Ligresti G, Aplin AC, Dunn BE, Morishita A, Nicosia RF. The acute phase reactant orosomucoid-1 is a bimodal regulator of angiogenesis with time- and context-dependent inhibitory and stimulatory properties. PLoS One. 2012;7 (8):e41387
  • Sochacka J, Pawelczak B. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. Acta Pol Pharm. 2012 Jan-Feb;69 (1):161-6
  • Christiansen MS, Hesse D, Ekbom P, Hesse U, Damm P, Hommel E, Feldt-Rasmussen B, Mathiesen E. Increased urinary orosomucoid excretion predicts preeclampsia in pregnant women with pregestational type 1 diabetes. Diabetes Res Clin Pract. 2010 Jul;89 (1):16-21
  • Christiansen MS, Hommel E, Magid E, Feldt-Rasmussen B. Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes. Diabetologia. 2002 Jan;45 (1):115-20
  • Harley J, Roberts R, Joyce P, Mulder R, Luty S, Frampton C, Kennedy M. Orosomucoid influences the response to antidepressants in major depressive disorder. J Psychopharmacol. 2010 Apr;24 (4):531-5
  • Ligresti G, Aplin AC, Dunn BE, Morishita A, Nicosia RF. The acute phase reactant orosomucoid-1 is a bimodal regulator of angiogenesis with time- and context-dependent inhibitory and stimulatory properties. PLoS One. 2012;7 (8):e41387
  • Sochacka J, Pawelczak B. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. Acta Pol Pharm. 2012 Jan-Feb;69 (1):161-6
  • Christiansen MS, Hesse D, Ekbom P, Hesse U, Damm P, Hommel E, Feldt-Rasmussen B, Mathiesen E. Increased urinary orosomucoid excretion predicts preeclampsia in pregnant women with pregestational type 1 diabetes. Diabetes Res Clin Pract. 2010 Jul;89 (1):16-21
  • Christiansen MS, Hommel E, Magid E, Feldt-Rasmussen B. Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes. Diabetologia. 2002 Jan;45 (1):115-20
  • Harley J, Roberts R, Joyce P, Mulder R, Luty S, Frampton C, Kennedy M. Orosomucoid influences the response to antidepressants in major depressive disorder. J Psychopharmacol. 2010 Apr;24 (4):531-5
  • Ligresti G, Aplin AC, Dunn BE, Morishita A, Nicosia RF. The acute phase reactant orosomucoid-1 is a bimodal regulator of angiogenesis with time- and context-dependent inhibitory and stimulatory properties. PLoS One. 2012;7 (8):e41387
  • Sochacka J, Pawelczak B. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. Acta Pol Pharm. 2012 Jan-Feb;69 (1):161-6
  • Christiansen MS, Hesse D, Ekbom P, Hesse U, Damm P, Hommel E, Feldt-Rasmussen B, Mathiesen E. Increased urinary orosomucoid excretion predicts preeclampsia in pregnant women with pregestational type 1 diabetes. Diabetes Res Clin Pract. 2010 Jul;89 (1):16-21
  • Christiansen MS, Hommel E, Magid E, Feldt-Rasmussen B. Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes. Diabetologia. 2002 Jan;45 (1):115-20
  • Harley J, Roberts R, Joyce P, Mulder R, Luty S, Frampton C, Kennedy M. Orosomucoid influences the response to antidepressants in major depressive disorder. J Psychopharmacol. 2010 Apr;24 (4):531-5
  • Ligresti G, Aplin AC, Dunn BE, Morishita A, Nicosia RF. The acute phase reactant orosomucoid-1 is a bimodal regulator of angiogenesis with time- and context-dependent inhibitory and stimulatory properties. PLoS One. 2012;7 (8):e41387
  • Sochacka J, Pawelczak B. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. Acta Pol Pharm. 2012 Jan-Feb;69 (1):161-6
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